Rice Cultivar Designated &#39;CLJ01&#39;

ABSTRACT

Provided are the herbicide-tolerant, aromatic (jasmine) rice cultivar designated ‘CLJ01’ and its hybrids and derivatives. The present invention provides a method for controlling weeds in the vicinity of rice, comprising contacting the rice with an herbicide, wherein said rice belongs to any of (a) variety ‘CLJ01’ or (b) a hybrid, derivative, or progeny of ‘CLJOV that expresses the imidazolinone herbicide resistance characteristics of ‘CLJOV. In some embodiments, the rice plants of the present invention include plants that comprise an AHAS mutant polypeptide that confers an increased tolerance to an imidazolinone herbicide when compared to a wild-type rice plant.

The benefit of the filing date of U.S. provisional application 62/662,446, filed 25 Apr. 2018, is claimed under 35 U.S.C. § 119(e) in the United States, and is claimed under applicable treaties and conventions in all countries.

TECHNICAL FIELD

This invention pertains to the herbicide-tolerant, jasmine rice cultivar designated ‘CLJ01,’ and to hybrids of, and cultivars derived from the rice cultivar designated ‘CLJ01.’

BACKGROUND ART

Rice is an ancient agricultural crop, and remains one of the world's principal food crops. There are two cultivated species of rice: Oryza sativa L., the Asian rice, and O. glaberrima Steud., the African rice. Oryza sativa L. constitutes virtually all of the world's cultivated rice and is the species grown in the United States. The three major rice-producing regions in the United States are the Mississippi Delta (Arkansas, Mississippi, northeast Louisiana, southeast Missouri), the Gulf Coast (southwest Louisiana, southeast Texas); and the Central Valley of California. See generally U.S. Pat. No. 6,911,589.

Rice is a semiaquatic crop that benefits from flooded soil conditions during part or all of the growing season. In the United States, rice is typically grown on flooded soil to optimize grain yields. Heavy clay soils or silt loam soils with hard pan layers about 30 cm below the surface are typical rice-producing soils, because they reduce water loss from soil percolation. Rice production in the United States can be broadly categorized as either dry-seeded or water-seeded. In the dry-seeded system, rice is sown into a well-prepared seed bed with a grain drill or by broadcasting the seed and incorporating it with a disk or harrow. Moisture for seed germination comes from irrigation or rainfall. Another method of dry-seeding is to broadcast the seed by airplane into a flooded field, and then promptly drain the water from the field. For the dry-seeded system, when the plants have reached sufficient size (four- to five-leaf stage), a shallow permanent flood of water 5 to 16 cm deep is applied to the field for the remainder of the crop season. Some rice is grown in upland production systems, without flooding.

One method of water-seeding is to soak rice seed for 12 to 36 hours to initiate germination, and then to broadcast the seed by airplane into a flooded field. The seedlings emerge through a shallow flood, or the water may be drained from the field for a short time to enhance seedling establishment. A shallow flood is then maintained until the rice approaches maturity. For both the dry-seeded and water-seeded production systems, the fields are drained when the crop is mature, and the rice is harvested 2 to 3 weeks later with large combines.

In rice breeding programs, breeders typically use the same production systems that predominate in the region. Thus, a drill-seeded breeding nursery is typically used by breeders in a region where rice is drill-seeded, and a water-seeded nursery is typically used in regions where water-seeding prevails.

Rice in the United States is classified into three primary market types by grain size, shape, and endosperm composition: long-grain, medium-grain, and short-grain. Typical U.S. long-grain cultivars cook dry and fluffy when steamed or boiled, whereas medium- and short-grain cultivars cook moist and sticky. Long-grain cultivars have been traditionally grown in the southern states and generally receive higher market prices in the U.S.

Aromatic rice has a subtle, nut-like aroma and taste, both while it is cooking and when it is eaten. Examples of aromatic rice include basmati rice, originally from India, and jasmine rice, originally from Thailand. Most rice varieties grown in the United States have traditionally been non-aromatic. There is a growing demand for aromatic rice in the United States and elsewhere.

Two recently-released varieties of aromatic rice were developed specifically for growing conditions in the southern United States: Jazzman, and Jazzman-2. See X. Sha et al., “Registration of ‘Jazzman’ Aromatic Long-Grain Rice,” J. Plant Reg., vol. 5, pp. 304-308 (2011); and X. Sha et al., “Registration of ‘Jazzman-2’ Aromatic Long-Grain Rice,” J. Plant Reg., vol. 7, pp. 26-30 (2012). These two varieties have a strong aroma and typical jasmine cooking qualities.

Both Jazzman and Jazzman-2 are “conventional” rice varieties, in the sense that they are not herbicide-tolerant. Because most rice fields in the southern United States (as well as many rice fields elsewhere) are infested with red rice and other types of weedy rice, it is difficult to avoid growing rice in fields that are not already infested with red rice. Jasmine-type rice, which is a specialty product, typically commands a premium price. However, contamination of the product with red rice reduces consumer appeal and can substantially lower the selling price that a grower might otherwise receive. There is an unfilled need for jasmine-type rice varieties that are well-suited for growing conditions in the southern United States and that are herbicide tolerant.

Although specific breeding objectives vary somewhat in different regions, increasing yield is a primary objective in all programs. Grain yield depends, in part, on the number of panicles per unit area, the number of fertile florets per panicle, and grain weight per floret. Increases in any or all of these components may help improve yields. Heritable variation exists for each of these components, and breeders may directly or indirectly select for any of them.

There are numerous steps in the development of any novel, desirable plant germplasm. Plant breeding begins with the analysis and definition of problems and weaknesses of the current germplasm, the establishment of program goals, and the definition of specific breeding objectives. The next step is selection (or generation) of germplasm that possesses the desired traits to meet the program goals. A goal is often to combine in a single variety an improved combination of desirable traits from two or more ancestral germplasm lines. These traits may include such things as higher seed yield, resistance to disease or insects, better stems and roots, tolerance to low temperatures, and better agronomic characteristics or grain quality.

The choice of breeding and selection methods depends on the mode of plant reproduction, the heritability of the trait(s) being improved, and the type of seed that is used commercially (e.g., F₁ hybrid, versus pure line or inbred cultivars). For highly heritable traits, a choice of superior individual plants evaluated at a single location may sometimes be effective, while for traits with low or more complex heritability, selection is often based on mean values obtained from replicated evaluations of families of related plants. Selection methods include pedigree selection, modified pedigree selection, mass selection, recurrent selection, and combinations of these methods.

The complexity of inheritance influences the choice of breeding method. Backcross breeding is used to transfer one or a few favorable genes for a highly heritable trait into a desirable cultivar. This approach has been used extensively for breeding disease-resistant cultivars. Various recurrent selection techniques are used to improve quantitatively-inherited traits controlled by numerous genes. The use of recurrent selection in self-pollinating crops depends on the ease of pollination, the frequency of successful hybrids from each pollination, and the number of hybrid offspring from each successful cross.

Promising advanced breeding lines are thoroughly tested and compared to appropriate standards in environments representative of the commercial target area(s), typically for three or more years. The best lines become candidates for new commercial cultivars; those still deficient in a few traits may be used as parents to produce new populations for further selection.

These processes, which lead ultimately to marketing and distribution of new cultivars or hybrids, typically take 8 to 12 years from the time of the first cross; they may further rely on (and be delayed by) the development of improved breeding lines as precursors. Development of new cultivars and hybrids is a time-consuming process that requires precise forward planning and efficient use of resources. There are never assurances of a successful outcome.

A particularly difficult task is the identification of individual plants that are, indeed, genetically superior. A plant's phenotype results from a complex interaction of genetics and environment. One method for identifying a genetically superior plant is to observe its performance relative to other experimental plants and to a widely grown standard cultivar raised in an identical environment. Repeated observations from multiple locations can help provide a better estimate of genetic worth.

The goal of rice breeding is to develop new, unique, and superior rice cultivars and hybrids. The breeder initially selects and crosses two or more parental lines, followed by self-pollination and selection, producing many new genetic combinations. The breeder can generate billions of different genetic combinations via crossing, selfing, and mutation breeding. The traditional breeder has no direct control of genetics at the molecular level. Therefore, two traditional breeders working independently of one another will never develop the same line, or even very similar lines, with the same traits.

Each year, the plant breeder selects germplasm to advance to the next generation. This germplasm is grown under different geographical, climatic, and soil conditions. Further selections are then made, during and at the end of the growing season. The resulting cultivars (or hybrids) and their characteristics are inherently unpredictable. This is because the traditional breeder's selection occurs in unique environments, with no control at the molecular level, and with potentially billions of different possible genetic combinations being generated. A breeder cannot predict the final resulting line, except possibly in a very gross and generic fashion. Further, the same breeder may not produce the same cultivar twice, even starting with the same parental lines, using the same selection techniques. This uncontrollable variation results in substantial effort and expenditures in developing superior new rice cultivars (or hybrids); and makes each new cultivar (or hybrid) novel and unpredictable.

The selection of superior hybrid crosses is conducted slightly differently. Hybrid seed is typically produced by manual crosses between selected male-fertile parents or by using genetic male sterility systems. These hybrids are typically selected for single gene traits that unambiguously indicate that a plant is indeed an F₁ hybrid that has inherited traits from both presumptive parents, particularly the male parent (since rice normally self-fertilizes). Such traits might include, for example, a semi dwarf plant type, pubescence, awns, or apiculus color. Additional data on parental lines, as well as the phenotype of the hybrid, influence the breeder's decision whether to continue with a particular hybrid cross or an analogous cross, using related parental lines.

Pedigree breeding and recurrent selection breeding methods are sometimes used to develop cultivars from breeding populations. These breeding methods combine desirable traits from two or more cultivars or other germplasm sources into breeding pools from which cultivars are developed by selfing and selection of desired phenotypes. The new cultivars are evaluated to determine commercial potential.

Pedigree breeding is often used to improve self-pollinating crops. Two parents possessing favorable, complementary traits are crossed to produce F₁ plants. An F₂ population is produced by selfing one or more F₁s. Selection of the superior individual plants may begin in the F₂ (or later) generation. Then, beginning in the F₃ (or other subsequent) generation, individual plants are selected. Replicated testing of panicle rows from the selected plants can begin in the F₄ (or other subsequent) generation, both to fix the desired traits and to improve the effectiveness of selection for traits that have low heritability. At an advanced stage of inbreeding (e.g., F₆ or F₇), the best lines or mixtures of phenotypically-similar lines are tested for potential release as new cultivars.

Mass and recurrent selection methods can also be used to improve populations of either self- or cross-pollinating crops. A genetically variable population of heterozygous individuals is either identified or created by intercrossing several different parents. The best offspring plants are selected based on individual superiority, outstanding progeny, or excellent combining ability. The selected plants are intercrossed to produce a new population in which further cycles of selection are continued.

Backcross breeding is often used to transfer genes for a simply inherited, highly heritable trait into a desirable homozygous cultivar or inbred line, which is the recurrent parent. The source of the trait to be transferred is called the donor parent. The resulting plant should ideally have the attributes of the recurrent parent (e.g., cultivar) and the desired new trait transferred from the donor parent. After the initial cross, individuals possessing the desired donor phenotype (e.g., disease resistance, insect resistance, herbicide tolerance) are selected and repeatedly crossed (backcrossed) to the recurrent parent.

The single-seed descent procedure in the strict sense refers to planting a segregating population, harvesting a sample of one seed per plant, and using the one-seed sample to plant the next generation. When the population has been advanced from the F₂ generation to the desired level of inbreeding, the several plants from which the lines are derived will each trace to different F₂ individuals. The number of plants in a population declines each generation, due to failure of some seeds to germinate or the failure of some plants to produce at least one seed. As a result, not all of the F₂ plants originally sampled in the population will be represented by progeny in subsequent generations.

In a multiple-seed procedure, the breeder harvests one or more seeds from each plant in a population and threshes them together to form a bulk. Part of the bulk is used to plant the next generation and part is held in reserve. The procedure has been referred to as modified single-seed descent or the pod-bulk technique. The multiple-seed procedure has been used to save labor at harvest. It is considerably faster to thresh panicles by machine than to remove one seed from each by hand as in the single-seed procedure. The multiple-seed procedure also makes it possible to plant the same number of seeds from a population for each generation of inbreeding. Enough seeds are harvested to compensate for plants that did not germinate or produce seed.

Other common and less-common breeding methods are known and used in the art. See, e.g., R. W. Allard, Principles of Plant Breeding (John Wiley and Sons, Inc., New York, N.Y., 1967); N. W. Simmonds, Principles of Crop Improvement (Longman, London, 1979); J. Sneep et al., Plant Breeding Perspectives (Pudoc, Wageningen, 1979); and W. R. Fehr, Principles of Cultivar Development: Theory and Technique (Macmillan Pub., New York, N.Y., 1987).

Proper testing should detect any major faults and establish the level of superiority or improvement over current cultivars. In addition to showing superior performance, there must be a demand for a new cultivar or hybrid; i.e., the new cultivar or hybrid should either be compatible with industry standards, or it should create a new market. The introduction of a new cultivar or hybrid may incur additional costs to the seed producer, the grower, processor, and consumer for such things as special advertising and marketing, altered seed and commercial production practices, and new product utilization. The testing that precedes the release of a new cultivar or hybrid should take into account research and development costs, in addition to technical superiority of the final cultivar or hybrid.

See, e.g., U.S. Pat. Nos. 5,545,822; 5,736,629; 5,773,703; 5,773,704; 5,952,553; 6,274,796; 6,943,280; 7,019,196; 7,345,221; 7,399,905; 7,495,153; 7,754,947; 7,786,360; 8,598,080; 8,841,525; 8,841,526; 8,946,528; 9,029,642; 9,090,904; and 9,220,220. These herbicide-tolerant rice plants are resistant to or tolerant of herbicides that normally inhibit the growth of rice plants. Thus, rice growers now can control weeds that previously were difficult to control in rice fields, including “red rice.” “Red rice” is a weedy relative of cultivated rice, and had previously been difficult to control because it actually belongs to the same genus (Oryza), and sometimes even the same species (O. sativa) as cultivated rice. Only recently, when herbicide tolerant rice became available, did it become possible to control red rice with herbicides in fields where cultivated rice was growing contemporaneously. There are currently only a limited number of herbicide-tolerant rice cultivars and hybrids available commercially. There is a continuing need for new herbicide-tolerant cultivars and hybrids—that is, rice plants that not only express a desired herbicide-tolerant phenotype, but that also possess other agronomically desirable characteristics. Additional herbicide-tolerant cultivars and hybrids will provide rice growers greater flexibility in planting and managing crops.

The inventors and their collaborators have publicly mentioned work with ‘CLJ01’ at LSU-sponsored growers meetings and field days in January, May, June, and July 2017; and January 2018. However, no one was given access to or samples of the plants or seeds on any of those occasions.

See also A. Famoso et al., “Registration of ‘CLJ01’ Rice,” Journal of Plant Registrations, doi:10.3198/jpr2018.06.0035crc (published March 2019); Schultz, “LSU AgCenter releases new rice hybrid,” press release (Mar. 21, 2018); 108^(th) Annual Research Report, H. Rouse Caffey Rice Research Station 2016, p. 266 (published April 2017); 109th Annual Research Report, H. Rouse Caffey Rice Research Station 2017, pp. 42, 51, 67 (published May 2018); and 110th Annual Research Report, H. Rouse Caffey Rice Research Station 2018, pp. 28-32, 81, 104 161-162, 185-186, 209-210, 223-224, 237-238, 257-258, 285-287, 299-301, 400 2018 (published April 2019).

DISCLOSURE OF THE INVENTION

We have discovered a novel, herbicide-resistant, high yielding, early maturing, semi-dwarf, long-grain aromatic (jasmine) rice cultivar designated ‘CLJ01’ (former experimental designation LA1602088). ‘CLJ01’ is the first aromatic, herbicide-tolerant rice cultivar to be commercially released. ‘CLJ01’ has higher yield and higher quality than the conventional aromatic lines Jazzman-1 and Jazzman-2. Over three years of tests at various locations, on average the yield has been 30 percent or more higher than that of Jazzman-2. The aroma is comparable to that of conventional Thai jasmine varieties. The quality is very high, with very low levels of chalk. ‘CLJ01’ has typical southern long grain cereal chemistry quality and cooking characteristics.

This invention also pertains to methods for producing a hybrid or new variety by crossing the rice variety ‘CLJ01’ with another rice line, one or more times. Thus any such methods using the rice variety ‘CLJ01’ are aspects of this invention, including backcrossing, hybrid production, crosses to populations, and other breeding methods involving ‘CLJ01.’ Hybrid plants produced using the rice variety ‘CLJ01’ as a parent are also within the scope of this invention. Optionally, either parent can, through routine manipulation of cytoplasmic or other factors through techniques known in the art, be produced in a male-sterile form.

In another embodiment, this invention allows for single-gene converted plants of ‘CLJ01.’ The single transferred gene may be a dominant or recessive allele. Preferably, the single transferred gene confers a trait such as resistance to insects; resistance to one or more bacterial, fungal or viral diseases; male fertility or sterility; enhanced nutritional quality; enhanced processing qualities; or an additional source of herbicide resistance. The single gene may be a naturally occurring rice gene or a transgene introduced through genetic engineering techniques known in the art. The single gene also may be introduced through traditional backcrossing techniques or genetic transformation techniques known in the art.

In another embodiment, this invention provides regenerable cells for use in tissue culture of rice plant ‘CLJ01.’ The tissue culture may allow for regeneration of plants having physiological and morphological characteristics of rice plant ‘CLJ01’ and of regenerating plants having substantially the same genotype as rice plant ‘CLJ01.’ Tissue culture techniques for rice are known in the art. The regenerable cells in tissue culture may be derived from sources such as embryos, protoplasts, meristematic cells, callus, pollen, leaves, anthers, root tips, flowers, seeds, panicles, or stems. In addition, the invention provides rice plants regenerated from such tissue cultures.

In another embodiment, the present invention provides a method for controlling weeds in the vicinity of rice. The method comprises contacting the rice with a herbicide, wherein said rice belongs to any of (a) variety ‘CLJ01’ or (b) a hybrid, derivative, or progeny of ‘CLJ01’ that expresses the imidazolinone herbicide resistance characteristics of ‘CLJ01.’

In some embodiments, the herbicide is an imidazolinone herbicide, a sulfonylurea herbicide, or a combination thereof.

In one embodiment, the rice is a rice plant and said contacting comprises applying the herbicide in the vicinity of the rice plant.

In another embodiment, the herbicide is applied to weeds in the vicinity of the rice plant.

In still further embodiments, the rice is a rice seed and said contacting comprises applying the herbicide to the rice seed.

In some embodiments, the present invention provides a method for treating rice. The method comprises contacting the rice with an agronomically acceptable composition, wherein said rice belongs to any of (a) variety ‘CLJ01’ or (b) a hybrid, derivative, or progeny of ‘CLJ01’ that expresses the imidazolinone herbicide resistance characteristics of ‘CLJ01.’

In one embodiment, the agronomically acceptable composition comprises at least one agronomically acceptable active ingredient.

In another embodiment, the agronomically acceptable active ingredient is selected from the group consisting of fungicides, insecticides, antibiotics, stress tolerance-enhancing compounds, growth promoters, herbicides, molluscicides, rodenticides, animal repellants, and combinations thereof.

In some embodiments, the rice plants of the present invention include plants that comprise an AHASL polypeptide (acetohydroxyacid synthase large subunit) having, relative to the wild-type AHASL polypeptide, an asparagine (N) at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, wherein such a plant has increased tolerance to an imidazolinone herbicide when compared to a wild-type rice plant.

Amino acid position 653 of Arabidopsis thaliana AHASL corresponds to amino acid position 627 of Oryza sativa AHASL. In the wild-type rice AHASL polypeptide, this position is a serine.

In other embodiments, the rice plants of the present invention include plants that comprise an AHASL polypeptide having a full-length, mature AHASL sequence variant, wherein there is an asparagine at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position and (ii) one or more conservative substitutions at one or more non-essential amino acid residues.

In one embodiment, the full-length, mature AHASL sequence variant has, over the full-length of the variant, at least about 95%, illustratively, at least about: 95%, 96%, 97%, 98%, 99%, 99.5%, and 99.9% sequence identity to the wild type, aside from the serine-asparagine substitution described above.

In some embodiments, the present invention provides a progeny rice line or variety obtainable from rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, said line ‘CLJ01’ having been produced by a process comprising:

(a) providing a rice seed of the Cypress variety (USDA ARS GRIN NPGS Accession No. PI 561734); and

(b) mutagenizing said rice seed to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of the Cypress rice, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and further breeding the altered plant,

wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of plants of said Cypress variety, and plants of said line ‘CLJ01’ and plants of said progeny line or variety contain said AHASL gene and exhibit said increased tolerance.

In other embodiments, the present invention provides a progeny rice line or variety obtained from rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, said line ‘CLJ01’ having been produced by a process comprising:

(a) providing a rice seed of the Cypress variety (USDA ARS GRIN NPGS Accession No. PI 561734); and

(b) mutagenizing said rice seed to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of the Cypress rice, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and further breeding the altered plant, wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of plants of said Cypress variety, and plants of said line ‘CLJ01’ and plants of said progeny line or variety contain said AHASL gene and exhibit said increased tolerance.

In some embodiments, the present invention provides a progeny rice plant obtainable from rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, said line ‘CLJ01’ having been produced by a process comprising:

(a) providing a rice seed of the Cypress variety (USDA ARS GRIN NPGS Accession No. PI 561734); and

(b) mutagenizing said rice seed to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of the Cypress rice, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and further breeding the altered plant,

wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of plants of said Cypress variety, and plants of said line ‘CLJ01’ and said progeny rice plant comprise said AHASL gene and exhibit said increased tolerance.

In some embodiments, the present invention provides a progeny rice plant obtained from rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, said line ‘CLJ01’ having been produced by a process comprising:

(a) providing a rice seed of the Cypress variety (USDA ARS GRIN NPGS Accession No. PI 561734); and

(b) mutagenizing said rice seed to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of the Cypress rice, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and further breeding the altered plant,

wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of plants of said Cypress variety, and plants of said line ‘CLJ01’ and said progeny rice plant comprise said AHASL gene and exhibit said increased tolerance.

In another embodiment, the present invention provides a progeny rice plant of rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, the progeny rice plant being obtainable by a process comprising:

(a) providing a plant of line ‘CLJ01,’ or tissue, seed, or cell thereof; and

(b) mutagenizing or transforming said plant, tissue, seed, or cell of step (a) to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of a wild-type rice plant, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and optionally further breeding the altered plant,

wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of the wild-type rice plant.

In another embodiment, the present invention provides a progeny rice plant of rice line ‘CLJ01,’ a representative sample of seeds of said line ‘CLJ01’ having been deposited under ATCC Accession No. PTA-125053, the progeny rice plant being obtained by a process comprising:

(a) providing a plant of line ‘CLJ01,’ or tissue, seed, or cell thereof; and

(b) mutagenizing or transforming said plant, tissue, seed, or cell of step (a) to produce an altered plant that contains in its genome an AHASL gene encoding an AHASL polypeptide having, relative to the wild-type AHASL polypeptide of a wild-type rice plant, an asparagine (N) substitution at amino acid position 653 (Arabidopsis thaliana AHASL numbering) or equivalent position, and optionally further breeding the altered plant,

wherein said altered plant of step (b) exhibits, upon expression of said AHASL gene, an increased tolerance to an imidazolinone herbicide as compared to that of the wild-type rice plant.

In other embodiments, the present invention provides a method for controlling weeds in a field, said method comprising: growing, in a field, a plant according to the present invention; and contacting said plant and weeds in the field with an effective amount of an AHAS-inhibiting herbicide to which the plant is tolerant, thereby controlling the weeds.

In some embodiments, improved rice plants and rice lines having tolerance to at least one AHAS-inhibitor herbicide are provided. In some embodiments, the AHAS-inhibitor herbicide is an imidazolinone herbicide. In some embodiments, the imidazolinone herbicide is imazethapyr, imazaquin, imazapyr, imazamox, or combinations thereof. Several examples of commercially available imidazolinone herbicides are, without limitation, PURSUIT® (imazethapyr), SCEPTER® (imazaquin), ARSENAL® (imazapyr), and Raptor™ Herbicide (imazamox). In some embodiments, the AHAS-inhibitor herbicide is a sulfonylurea herbicide. In one embodiment, the sulfonylurea herbicide is nicosulfuron.

The rice plants and rice lines of the present invention also provide for improved systems and methods for controlling weeds using at least one AHAS-inhibitor herbicide. In some embodiments, the AHAS-inhibitor herbicide is an imidazolinone herbicide. In some embodiments, the imidazolinone herbicide is imazethapyr, imazaquin, imazapyr, imazamox, or combinations thereof. In some embodiments, the AHAS-inhibitor herbicide is a sulfonylurea herbicide. In one embodiment, the sulfonylurea herbicide is nicosulfuron.

In some embodiments, the AHAS-inhibitor herbicide is an imidazolinone herbicide, a sulfonylurea herbicide, or combinations thereof.

Definitions

The following definitions apply throughout the specification and claims, unless context clearly indicates otherwise:

“Days to 50% heading.” Average number of days from seeding to the day when 50% of all panicles are exerted at least partially through the leaf sheath. A measure of maturity.

“Grain Yield.” Grain yield is measured in pounds per acre, at 12.0% moisture. Grain yield depends on a number of factors, including the number of panicles per unit area, the number of fertile florets per panicle, and grain weight per floret.

“Lodging Percent.” Lodging is a subjectively measured rating, and is the percentage of plant stems leaning or fallen completely to the ground before harvest.

“Grain Length (L).” Length of a rice grain, or average length, measured in millimeters.

“Grain Width (W).” Width of a rice grain, or average width, measured in millimeters.

“Length/Width (L/W) Ratio.” This ratio is determined by dividing the average length (L) by the average width (W).

“1000 Grain Wt.” The weight of 1000 rice grains, measured in grams.

“Harvest Moisture.” The percentage moisture in the grain when harvested.

“Plant Height.” Plant height in centimeters, measured from soil surface to the tip of the extended panicle at harvest.

“Apparent Amylose Percent.” The percentage of the endosperm starch of milled rice that is amylose. The apparent amylose percent is an important grain characteristic that affects cooking behavior. Standard long grains contain 20 to 23 percent amylose. Rexmont-type long grains contain 24 to 25 percent amylose. Short and medium grains contain 13 to 19 percent amylose. Waxy rice contains zero percent amylose. Amylose values, like most characteristics of rice, depend on the environment. “Apparent” refers to the procedure for determining amylose, which may also involve measuring some long chain amylopectin molecules that bind to some of the amylose molecules. These amylopectin molecules actually act similar to amylose in determining the relative hard or soft cooking characteristics.

“Alkali Spreading Value.” An index that measures the extent of disintegration of the milled rice kernel when in contact with dilute alkali solution. It is an indicator of gelatinization temperature. Standard long grains have a 3 to 5 Alkali Spreading Value (intermediate gelatinization temperature).

“Peak Viscosity.” The maximum viscosity attained during heating when a standardized, instrument-specific protocol is applied to a defined rice flour-water slurry.

“Trough Viscosity.” The minimum viscosity after the peak, normally occurring when the sample starts to cool.

“Final Viscosity.” Viscosity at the end of the test or cold paste.

“Breakdown.” The peak viscosity minus the hot paste viscosity.

“Setback.” Setback 1 is the final viscosity minus the trough viscosity. Setback 2 is the final viscosity minus the peak viscosity.

“RVA Viscosity.” Viscosity, as measured by a Rapid Visco Analyzer, a widely used laboratory instrument to examine the paste viscosity or thickening ability of milled rice during the cooking process.

“Hot Paste Viscosity.” Viscosity measure of rice flour/water slurry after being heated to 95° C. Lower values indicate softer and stickier cooking types of rice.

“Cool Paste Viscosity.” Viscosity measure of rice flour/water slurry after being heated to 95° C. and uniformly cooled to 50° C. Values less than 200 indicate softer cooking types of rice.

“Allele.” An allele is any of one or more alternate forms of the same gene. In a diploid cell or organism such as rice, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

“Backcrossing.” Backcrossing is a process in which a breeder repeatedly crosses hybrid progeny back to one of the parents, for example, crossing a first generation hybrid F with one of the parental genotypes of the F₁ hybrid, and then crossing a second generation hybrid F₂ with the same parental genotype, and so forth.

“Essentially all the physiological and morphological characteristics.” A plant having “essentially all the physiological and morphological characteristics” of a specified plant refers to a plant having the same general physiological and morphological characteristics, except for those characteristics that are derived from a particular converted gene.

“Quantitative Trait Loci (QTL).” Quantitative trait loci (QTL) refer to genetic loci that to some degree control numerically measurable traits, generally traits that are continuously distributed.

“Regeneration.” Regeneration refers to the development of a plant from tissue culture.

“Single Gene Converted (Conversion).” Single gene converted (conversion) includes plants developed by backcrossing, wherein essentially all of the desired morphological and physiological characteristics of a parental variety are recovered, while also retaining a single gene that is transferred into the plants via crossing and backcrossing. The term can also refer to the introduction of a single gene through genetic engineering techniques known in the art.

MODES FOR CARRYING OUT THE INVENTION

‘CLJ01’ (earlier experimental designation LA1602088) is a semidwarf, aromatic (jasmine) long-grain Clearfield rice line with excellent grain yield and quality. It was developed using a pedigree selection system at the Louisiana State University Agricultural Center Rice Research Station (RRS) in Crowley, La. ‘CLJ01’ was selected from the cross JZMN/08CLR004//RU0802146/3/RU1002146, which was made at the H. Rouse Caffey Rice Research Station (RRS) in 2010. The line was derived from a single panicle selection from an F₃ row (13-22709) made at the RRS in 2013. ‘CLJ01’ was evaluated in the Clearfield Jazzman Preliminary Yield Trial at the Rice Research Station in 2015 with the experimental designation CLPYJ027 before being entered into the three multi-location yield tests, including the Cooperative Uniform Regional Rice Nurseries (URN), the Clearfield Multi-location test, and the Clearfield Jazzman test in 2016 under the designation RU1602088.

JZMN is the publicly-released variety ‘Jazzman.’

08CLR004 is an imazethapyr-resistant experimental line, never released as a commercial variety. The pedigree of 08CLR004 is 9502008-A/Drew//CLR20. 9502008-A is a sister line to the variety ‘Cocodrie,’ never released. ‘Drew’ is a variety released by the University of Arkansas. CLR20 is an advanced Clearfield experimental line developed at Louisiana State University through a marker-assisted selection program, never released.

RU0802146 and RU1002146 are sister lines. Both are experimental lines developed at the RRS in Crowley, La., never released. Both lines have the pedigree NEWBONNET/KATY//9902207x2/3/JAZZMAN/DELLA//LEAH/DELLA. Newbonnet and Katy are released varieties from the University of Arkansas. Jazzman is an LSU AgCenter RRS variety, as discussed above. Della and Leah are released varieties from the LSU AgCenter RRS. 9902207 was an experimental line from the LSU AgCenter RRS, never released.

The herbicide resistance characteristics of ‘CLJ01’ are essentially identical by virtue of common ancestry to the herbicide resistance characteristics of the variety ‘CL161’ (ATCC deposit PTA-904), which is also known as line PWC16 as disclosed by U.S. Pat. Nos. 6,943,280 and 7,019,196, each of which is incorporated herein by reference in its entirety. ‘CL161’ is a herbicide-tolerant variety derived at the Louisiana Rice Research Station by mutation breeding from the original variety ‘Cypress.’ Further, U.S. Pat. No. 6,943,280 discloses that in the AHAS enzyme DNA sequence of line PWC16, the codon corresponding to amino acid 627 is AAT, which encodes asparagine, versus AGT (serine) for the wild-type, and that this serine-to-asparagine substitution is believed to be responsible for the herbicide resistance displayed by the AHAS enzyme of line PWC16. See, e.g., the 19th sequence listing in the 6,943,280 patent. ‘CLJ01’ and its hybrids and derived varieties are adapted for growing throughout the rice growing areas of Louisiana, Texas, Arkansas, Mississippi and Missouri; and will also be well suited for growing in many other rice-producing areas throughout the world.

After the initial cross was made, the line was harvested and selected through early generations for phenotypic superiority for characteristics such as short plant architecture, grain shape and uniformity, seedling vigor, tiller number, and grain size. In later generations (during seed increase), the line was selected for uniformity and purity both within and between panicle rows. Variants removed from ‘CLJ01’ seed-increase fields were primarily taller or later plants. Other variants removed included those with any one or more of the following: leaf pubescence, earlier, shorter, medium grain, intermediate grain, gold hull, and lighter colored leaf. The overall incidence of variants was less than 1 per 5,000 plants. Foundation seed rice was grown, beginning with the F₇ generation. Seed from the F₅, F₆, and F₇ generations was entered into an experimental line testing program, and was also tested at several locations in Louisiana rice producing areas. ‘CLJ01’ has been observed to be stable for at least three generations.

TABLE A Origin and Breeding History Year Generation Test (Entry #) 2010 F0 BCS019/10HB175 2011 F1 11TS280 2012 F2 F7670-7671 2013 F3 13-22709 2014 F4 14-13563 (panicle row increase) 2015 F5 CLPYJ 027; 15-57615 (panicle row increase) 2016 F6 URN088, CLR009, CLJ027 (panicle row increase) 2017 F7 URN174, CA243, CLR 012, CLPR 436. CLTX 019(TEST 1) (Breeder/Foundation seed) DP003, CLPY 372(TEST 1), CLPY 747(TEST 2) (Breeder/Foundation seed)

‘CLJ01’ averaged 38 inches (97 cm) in height in yield tests across Louisiana, which is the same height as CL151 and one inch (2 cm) taller than CL111 and CL153. At 85 days to 50% heading, it is three days later than CL111 and CL153 and one day later than CL151.

The leaves, lemma, and palea of LA1602088 are glabrous. The spikelet and apiculus are straw-colored. The grain is aromatic.

‘CLJ01’ has a Jazzman-grain cooking quality, with low amylose content and a low gelatinization temperature. The average amylose content of ‘CLJ01’ is 13.0, compared with 23.5 for CL151, 22.4 for CL111, and 22.8 for CL153. The average alkali spread value of ‘CLJ01,’ CL111, CL151, and CL153 are 6.2, 4.2, 4.0 and 4.2, respectively.

‘CLJ01’ is moderately susceptible to blast, and is susceptible to sheath blight and bacterial panicle blight.

Variants observed and removed from increase fields of ‘CLJ01’ included any combination of the following: pubescent, taller, shorter, later, earlier, short-, medium- and intermediate-grain types, gold, purple and black hull and sterile panicle. The total numbers of variants were fewer than 1 per 5000 plants.

VARIETY DESCRIPTION INFORMATION

Rice cultivar ‘CLJ01’ was observed to possess the following morphological and other characteristics, based on averages of tests conducted at multiple over several growing seasons; data for other varieties are shown for comparison:

TABLE B Data Summary Performance Number Trait CLJ01 CL111 CL151 CL153 of Tests Reference Yield 7282 7691 8107 7906 40 Table 2 Whole 67.6 64.5 63.5 62.3 32 Table 4 Total 73.3 73.3 72.3 71.6 32 Table 6 Length-Rough (mm) 9.13 9.34 8.74 9.51 2 Table 18 Width-Rough (mm) 2.58 2.61 2.61 2.42 2 Table 18 L/W Ratio-Rough 3.54 3.58 3.35 3.92 2 Table 18 Thickness-Rough (mm) 1.90 1.96 2.07 2.05 2 Table 18 Weight-Rough (g) 22.4 26.9 24.5 25.3 2 Table 18 Length-Brown (mm) 7.11 7.31 6.68 7.26 2 Table 18 Width-Brown (mm) 2.33 2.27 2.22 2.10 2 Table 18 L/W Ratio-Brown 3.05 3.22 3.01 3.45 2 Table 18 Thickness-Brown (mm) 1.52 1.74 1.75 1.81 2 Table 18 Weight-Brown (g) 18.0 22.3 19.5 22.2 2 Table 18 Length-Milled (mm) 6.85 6.45 6.36 6.95 2 Table 18 Width-Milled (mm) 2.25 2.15 2.14 2.01 2 Table 18 L/W Ratio-Milled 3.04 3.00 2.97 3.45 2 Table 18 Thickness-Milled (mm) 1.46 1.68 1.66 1.71 2 Table 18 Weight-Milled (g) 17.2 19.3 17.8 19.3 2 Table 18 Vigor 4 4 4 3 32 Table 8 Height 38 37 38 37 40 Table 10 Days to 50% 85 82 84 82 36 Table 12 Blast 1.3 4.0 2.2 1.7 3 Table 13 Bacterial Panicle Blight 3.0 2.8 2.2 2.0 2 Table 14 Cercospora 3.5 4.4 3.7 4.2 2 Table 15 Straighthead 4.7 4.8 3.9 4.7 2 Table 16

TABLE 1 Average main crop yields (lb/A) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 8060 8441 8622 na 2016 URN - LOUISIANA 7855 8797 9127 9388 URN - ARKANSAS 4476 7567 6795 6829 URN - MISSISSIPPI 8066 10032 8298 10240 URN - TEXAS 6050 6753 6263 7229 CL JZMN MULTI - RRS 8260 9544 9297 9398 CL JZMN MULTI - EVANGELINE 7751 8278 9400 8811 CL JZMN MULTI - LAKE ARTHUR 5996 5543 6766 7226 CL JZMN - RICHLAND 6106 5635 5641 5865 CL MULTI - RRS 7856 9261 9474 9654 CL MULTI - EVANGELINE 8024 8371 8963 8719 CL MULTI - LAKE ARTHUR 6028 5063 7100 7337 CL MULTI - RICHLAND 5771 5853 6224 6345 2016 Average 6853 7558 7779 8087 Abbreviations: JZMN = Jazzman, CLPY = Clearfield Preliminary Yield Test, URN = Uniform Regional Nursery, CL MULTI = Clearfield test conducted at various locations, DATE OF PLANTING = series of tests in which the same line is planted on eight different dates approximately two weeks apart, RRS = Louisiana State University Rice Research Station (Crowley, LA) Note: (multiply lb/A by 1.121 to obtain kg/ha)

TABLE 2 Average main crop yields (lb/A) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 6690 7048 7931 7245 URN - ARKANSAS 6816 8527 8752 9497 URN - MISSISSIPPI 8558 9767 9797 10187 CA - RRS 8027 7830 8903 8523 CA - ACADIA 6763 6610 7494 7410 CA - EVANGELINE 6651 5547 7403 7271 CA - JEFF DAVIS 7546 7233 8693 7827 CA - LAKE ARTHUR 7384 6418 7795 7451 CA - ST. JOE 8128 8156 9168 8292 CA - ST. LANDRY 6478 5096 7589 7006 CL MULTI - RRS 6278 7885 8806 7973 CL MULTI - EVANGELINE 6615 6369 7488 7965 CL MULTI - LAKE ARTHUR 6596 7506 8619 8036 CLPY TEST 1 - RRS 7216 7567 8241 8345 CLPY TEST 2 - RRS 8005 7226 7763 8436 CLPY - TEXAS 9818 10671 11373 10393 CLPR - RRS 5690 5012 6688 6239 DATE OF PLANTING 1 - RRS 7433 7579 9150 7916 DATE OF PLANTING 2 - RRS 7476 8135 8680 7676 DATE OF PLANTING 3 - RRS 7293 8003 8469 8342 DATE OF PLANTING 4 - RRS 6982 7878 7873 7968 DATE OF PLANTING 5 - RRS 6759 6007 6317 7303 DATE OF PLANTING 6 - RRS 4927 4871 6104 5775 DATE OF PLANTING 7 - RRS 5569 5781 5552 5253 DATE OF PLANTING 8 - RRS 4477 6532 5669 6791 DATE OF PLANTING 9 - RRS 6104 4698 5632 5712 2017 Average 6934 7075 7921 7724 2015-2017 Grand Average 7282 7691 8107 7906

TABLE 3 Whole rice yield (%) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 71.9 69.6 67.0 n/a 2016 URN - LOUISIANA 69.6 67.0 62.8 65.3 URN - ARKANSAS 57.1 62.5 62.8 54.3 URN - MISSISSIPPI 68.2 65.6 67.7 65.6 URN - TEXAS 62.0 57.0 59.0 63.0 CL JZMN MULTI - RRS 69.5 66.0 64.1 64.9 CL JZMN MULTI - EVANGELINE 69.4 66.6 65.9 66.7 CL JZMN MULTI - LAKE ARTHUR 64.1 54.2 56.2 59.6 CL MULTI - RRS 68.7 67.8 63.3 65.8 CL MULTI - EVANGELINE 52.3 49.8 52.2 54.7 CL MULTI - LAKE ARTHUR 64.5 57.3 57.1 57.0 2016 Average 64.6 61.4 61.1 61.7

TABLE 4 Whole rice yield (%) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 66.7 59.0 56.5 55.7 URN - ARKANSAS 66.0 61.3 62.7 62.0 URN - MISSISSIPPI 66.2 65.7 65.8 65.5 CA - RRS 69.7 61.3 62.0 55.5 CA - ACADIA 64.0 63.4 64.4 63.1 CA - LAKE ARTHUR 63.9 59.5 59.1 63.4 CL MULTI - RRS 65.9 57.7 65.5 65.2 CL MULTI - LAKE ARTHUR 66.8 62.6 60.7 62.1 CLPY TEST 1 - RRS 68.3 63.9 60.9 n/a CLPY TEST 2 - RRS 70.0 64.6 61.6 64.5 CLPR - RRS 67.4 62.8 61.5 61.0 DATE OF PLANTING 1 - RRS 69.3 68.3 65.7 67.0 DATE OF PLANTING 2 - RRS 59.6 67.5 56.0 67.7 DATE OF PLANTING 3 - RRS 67.3 62.3 64.7 65.6 DATE OF PLANTING 4 - RRS 69.1 65.9 66.0 64.4 DATE OF PLANTING 5 - RRS 66.5 61.7 58.1 65.2 DATE OF PLANTING 6 - RRS 65.3 65.8 62.6 62.5 DATE OF PLANTING 7 - RRS 66.1 63.2 65.7 62.6 DATE OF PLANTING 8 - RRS 67.4 49.9 61.1 55.5 DATE OF PLANTING 9 - RRS 59.5 64.8 69.1 64.8 2017 Average 66.3 62.6 62.5 62.8 2015-2017 Grand Average 67.6 64.5 63.5 62.3

TABLE 5 Total rice yield (%) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 75.3 75.7 73.4 na 2016 URN - LOUISIANA 71.3 74.9 72.5 72.0 URN - ARKANSAS 66.9 70.0 69.7 65.4 URN - MISSISSIPPI 71.3 71.5 71.9 70.6 URN - TEXAS 70.0 72.0 71.0 72.0 CL JZMN MULTI - RRS 74.0 73.1 72.6 71.6 CL JZMN MULTI - EVANGELINE 74.2 75.2 73.5 73.8 CL JZMN MULTI - LAKE ARTHUR 71.6 67.5 69.4 69.9 CL MULTI - RRS 73.2 73.4 70.7 72.9 CL MULTI - EVANGELINE 72.8 72.0 73.5 73.0 CL MULTI - LAKE ARTHUR 73.1 70.9 69.7 70.3 2016 Average 72.1 72.1 71.4 71.1

TABLE 6 Total rice yield (%) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 74.5 74.1 70.6 73.7 URN - ARKANSAS 71.4 70.5 69.5 69.6 URN-MISSISSIPPI 71.6 72.4 72.9 71.7 CA - RRS 78.0 76.5 76.0 75.0 CA - ACADIA 70.8 71.4 71.4 72.1 CA - LAKE ARTHUR 71.7 69.1 71.6 73.3 CL MULTI - RRS 71.6 74.1 73.3 72.1 CL MULTI - LAKE ARTHUR 74.6 72.9 72.2 72.9 CLPY TEST 1 - RRS 73.5 71.8 72.5 n/a CLPY TEST 2 - RRS 75.5 74.7 72.0 72.0 CLPR - RRS 71.8 66.9 68.7 69.1 DATE OF PLANTING 1 - RRS 72.9 72.9 72.4 72.1 DATE OF PLANTING 2 - RRS 73.3 72.8 71.5 73.3 DATE OF PLANTING 3 - RRS 71.3 72.1 72.3 71.6 DATE OF PLANTING 4 - RRS 72.2 71.0 71.7 71.1 DATE OF PLANTING 5 - RRS 70.5 70.9 67.9 71.3 DATE OF PLANTING 6 - RRS 70.0 71.4 73.0 71.2 DATE OF PLANTING 7 - RRS 70.9 71.4 73.0 71.2 DATE OF PLANTING 8 - RRS 73.8 73.5 74.1 72.9 DATE OF PLANTING 9 - RRS 72.9 73.7 73.9 73.7 2017 Average 72.6 72.2 72.0 72.1 2015-2017 Grand Average 73.3 73.3 72.3 71.6

TABLE 7 Seedling vigor for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 4 4 4 na 2016 URN - LOUISIANA 4 3 3 4 URN - ARKANSAS 3 3 3 3 CL JZMN MULTI - RRS 4 3 3 3 CL JZMN MULTI - EVANGELINE 4 4 5 4 CL JZMN MULTI - LAKE ARTHUR 3 3 4 3 CL MULTI - RRS 4 3 3 3 CL MULTI - EVANGELINE 5 4 4 5 CL MULTI - LAKE ARTHUR 3 3 4 3 2016 Average 4 3 4 4

TABLE 8 Seedling vigor for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 3 4 3 3 URN - ARKANSAS 3 3 4 3 CA - RRS 3 4 3 3 CA - ACADIA 3 5 4 4 CA - EVANGELINE 3 5 4 3 CA - JEFF DAVIS 4 7 5 5 CA - LAKE ARTHUR 5 6 5 4 CA - ST. JOE 4 5 4 3 CL MULTI - RRS 5 2 3 3 CL MULTI - EVANGELINE 4 3 3 3 CL MULTI - LAKE ARTHUR 5 4 3 4 CLPY TEST 1 - RRS 4 4 4 3 CLPY TEST 2 - RRS 4 4 5 3 CLPR - RRS 2 3 4 4 DATE OF PLANTING 1 - RRS 3 3 3 4 DATE OF PLANTING 2 - RRS 4 4 4 4 DATE OF PLANTING 3 - RRS 4 5 4 4 DATE OF PLANTING 4 - RRS 4 4 4 4 DATE OF PLANTING 5 - RRS 3 5 4 3 DATE OF PLANTING 6 - RRS 3 4 3 3 DATE OF PLANTING 7 - RRS 3 4 4 3 DATE OF PLANTING 8 - RRS 4 4 4 3 DATE OF PLANTING 9 - RRS 3 3 3 3 2017 Average 4 4 4 3 2015-2017 Grand Average 4 4 4 3

TABLE 9 Mean plant height (in) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 42 39 39 na 2016 URN - LOUISIANA 38 38 39 37 URN - ARKANSAS 44 45 43 44 URN - MISSISSIPPI 42 45 45 44 URN - TEXAS 33 35 33 35 CL JZMN MULTI - RRS 38 38 39 37 CL JZMN MULTI - EVANGELINE 37 36 39 38 CL JZMN MULTI - LAKE ARTHUR 33 33 37 35 CL JZMN MULTI - RICHLAND 35 36 38 39 CL MULTI - RRS 37 39 39 38 CL MULTI - EVANGELINE 36 36 38 37 CL MULTI - LAKE ARTHUR 33 31 37 34 CL MULTI - RICHLAND 35 35 38 38 2016 Average 37 37 39 38

TABLE 10 Mean plant height (in) for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 33 33 35 31 URN - ARKANSAS 36 39 41 40 URN - MISSISSIPPI 40 42 42 40 CA - RRS 33 35 36 35 CA - ACADIA 34 35 35 35 CA - EVANGELINE 34 34 34 35 CA - JEFF DAVIS 32 34 34 35 CA - LAKE ARTHUR 33 35 34 34 CA - ST. JOE 38 38 38 36 CA - ST. LANDRY 38 37 38 36 CL MULTI - RRS 36 36 40 38 CL MULTI - EVANGELINE 36 35 36 36 CL MULTI - LAKE ARTHUR 33 35 34 33 CLPY TEST 1 - RRS 34 35 35 33 CLPY TEST 2 - RRS 34 34 35 34 CLPY - TEXAS 36 36 36 37 CLPR - RRS 40 36 39 38 DATE OF PLANTING 1 - RRS 31 32 32 31 DATE OF PLANTING 2 - RRS 31 33 33 31 DATE OF PLANTING 3 - RRS 36 38 36 37 DATE OF PLANTING 4 - RRS 35 38 38 36 DATE OF PLANTING 5 - RRS 38 40 39 38 DATE OF PLANTING 6 - RRS 35 37 37 36 DATE OF PLANTING 7 - RRS 36 36 36 34 DATE OF PLANTING 8 - RRS 36 36 39 37 DATE OF PLANTING 9 - RRS 35 36 36 36 2017 Average 35 36 36 35 2015-2017 Grand Average 38 37 38 37

TABLE 11 Mean number of days to 50% heading for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CLPY JZMN 90 91 90 na 2016 URN - LOUISIANA 76 75 76 78 URN - ARKANSAS 86 85 85 87 URN - MISSISSIPPI 75 73 77 77 URN - TEXAS 72 86 87 81 CL JZMN MULTI - RRS 77 72 74 76 CL JZMN MULTI - EVANGELINE 79 71 77 80 CL JZMN MULTI - LAKE ARTHUR 88 76 86 86 CL MULTI - RRS 77 73 75 76 CL MULTI - EVANGELINE 79 71 78 78 CL MULTI - LAKE ARTHUR 87 76 83 83 2016 Average 81 77 81 80

TABLE 12 Mean number of days to 50% heading for CLJ01, CL111, CL151 and CL153 across several trials at multiple locations (2017). YEAR TEST CLJ01 CL111 CL151 CL153 2017 URN - LOUISIANA 82 78 79 80 URN - ARKANSAS 83 79 81 82 URN - MISSISSIPPI 93 91 90 91 CA - RRS 81 81 82 81 CA - ACADIA 88 79 84 85 CA - EVANGELINE 94 83 87 90 CA - JEFF DAVIS 86 85 86 87 CA - LAKE ARTHUR 87 83 83 84 CL MULTI - RRS 90 85 89 89 CL MULTI - EVANGELINE 96 81 89 89 CL MULTI - LAKE ARTHUR 87 79 82 84 CLPY TEST 1 - RRS 90 83 86 86 CLPY TEST 2 - RRS 88 82 86 85 CLPY - TEXAS 85 82 84 84 CLPR - RRS 76 67 74 75 DATE OF PLANTING 1 - RRS 101 96 96 101 DATE OF PLANTING 2 - RRS 86 81 85 86 DATE OF PLANTING 3 - RRS 86 82 83 84 DATE OF PLANTING 4 - RRS 75 75 76 77 DATE OF PLANTING 5 - RRS 88 80 82 83 DATE OF PLANTING 6 - RRS 83 72 78 76 DATE OF PLANTING 7 - RRS 75 72 74 74 DATE OF PLANTING 8 - RRS 74 68 73 73 DATE OF PLANTING 9 - RRS 72 61 69 67 2017 Average 85 79 82 83 2015-2017 Grand Average 85 82 84 82

TABLE 13 Reaction of CLJ01, CL111, CL151 and CL153 to blast (Pyricularia oryzae) (2015-2016). YEAR TEST CLJ01 CL111 CL151 CL153 2015 CL JZMN MULTI - RRS 1.5 2.0 3.5 na 2015 Average 1.5 2.0 3.5 na 2016 CL JZMN MULTI - LAKE ARTHUR 0.7 5.3 2.0 1.0 CL MULTI - LAKE ARTHUR 1.7 4.7 1.0 2.3 2016 Average 1.2 5.0 1.5 1.7 2015-2016 Grand Average 1.3 4.0 2.2 1.7 * Using a scale of 0 = very resistant to 9 = very susceptible.

TABLE 14 Reaction of CLJ01, CL111, CL151 and CL153 to bacterial panicle blight (Burkholderia glumae) (2016). YEAR TEST CLJ01 CL111 CL151 CL153 2016 CL JZMN MULTI - LAKE ARTHUR 2.3 3.0 1.7 1.3 CL MULTI - LAKE ARTHUR 3.7 2.7 2.7 2.7 2016 Average 3.0 2.8 2.2 2.0 * Using a scale of 0 = very resistant to 9 = very susceptible.

TABLE 15 Reaction of CLJ01, CL111, CL151 and CL153 to narrow brown leaf spot (Cercospora oryzae) (2016). YEAR TEST CLJ01 CL111 CL151 CL153 2016 CL JZMN MULTI - EVANGELINE 3.7 4.7 4.0 4.3 CL MULTI - EVANGELINE 3.3 4.0 3.3 4.0 2016 Average 3.5 4.4 3.7 4.2 * Using a scale of 0 = very resistant to 9 = very susceptible.

TABLE 16 Reaction of CLJ01, CL111, CL151 and CL153 to the physiological disorder straighthead (2016-2017). YEAR TEST CLJ01 CL111 CL151 CL153 2016 RRS 4.3 5.3 4.0 4.7 2017 RRS 5.0 4.3 3.7 4.7 2016-2017 Grand 4.7 4.8 3.9 4.7 Average

TABLE 17 2017 Crowley Disease Nursery YEAR DISEASE CLJ01 CL111 CL151 CL153 2017 SHEATH BLIGHT 6.7 7.7 6.9 7.2 BLAST 3.0 2.3 2.0 2.5 BACTERIAL 5.0 7.0 3.9 3.9 PANICLE BLIGHT

TABLE 18 Rough, brown and milled grain dimensions and weight of CLJ01, CL111, CL151 and CL153 grown in Crowley, LA. Length Width L/W Thick- Variety Type mm mm Ratio ness Weight CLJ01 Rough 9.13 2.58 3.54 1.90 22.4 Brown 7.11 2.33 3.05 1.52 18.0 Milled 6.85 2.25 3.04 1.46 17.2 CL111 Rough 9.34 2.61 3.58 1.96 26.9 Brown 7.31 2.27 3.22 1.74 22.3 Milled 6.45 2.15 3.00 1.68 19.3 CL151 Rough 8.74 2.61 3.35 2.07 24.5 Brown 6.68 2.22 3.01 1.75 19.5 Milled 6.36 2.14 2.97 1.66 17.8 CL153 Rough 9.51 2.42 3.92 2.05 25.3 Brown 7.26 2.10 3.45 1.81 22.2 Milled 6.95 2.01 3.45 1.71 19.3

TABLE 19 2AP aroma values of CLJ01 and experimental lines from various tests, RRS 2015 Analysis TEST REP 1 REP 2 REP 3 AVERAGE DEC. 1, CLJ01 4.48 4.23 4.02 4.24 2015 CLPYJ 002 4.18 3.95 3.76 3.96 CLPYJ 010 4.87 5.11 5.06 5.01 CLPYJ 011 4.00 4.05 3.93 3.99 CLPYJ 017 4.75 5.21 4.88 4.95 CLPYJ 020 4.12 3.90 4.16 4.06 CLPYJ 023 3.36 3.28 3.32 3.32 CLPYJ 031 3.50 3.46 3.20 3.39 CLPYJ 034 6.53 6.28 6.10 6.30 CLPYJ 002 4.37 4.55 4.16 4.36 CLPYJ 038 4.89 4.63 4.80 4.77 CLPYJ 040 4.34 4.02 4.05 4.14 CLPYJ 042 4.15 4.42 4.06 4.21 CLPYJ 043 4.12 3.92 3.90 3.98 KDML105 (CHECK) 4.12 3.92 3.90 3.98

TABLE 20 2AP aroma values of CLJ01. Jazzman and Jazzman2 from various tests, RRS 2016 (2AP = 2-acetyl-1-pyrroline, as measured by gas chromatography, the compound that imparts aromatic characteristics to rice) Analysis TEST REP 1 REP 2 REP 3 AVERAGE NOV. 2, CLJ01 2.891 3.222 3.243 3.119 2016 Jazzman 2.228 2.373 2.496 2.365 Jazzman2 4.189 4.370 4.820 4.460 KDML105 3.121 n/a n/a 3.121 JUN. 13, CLJ01 2.392 2.567 2.546 2.502 2016 Jazzman 1.061 0.985 1.088 1.045 KDML105 (CHECK) 2.533 2.226 2.100 2.286

TABLE 21 2AP aroma values of CLJ01 and experimental lines from various tests, RRS 2017 Analysis TEST REP 1 REP 2 REP 3 AVERAGE SEP. 18, CLJ01 FAMILY 1 2.720 2.649 2.572 2.647 2017 CLJ01 FAMILY 2 2.679 2.527 2.344 2.517 CLJ01 FAMILY 3 2.719 2.717 2.484 2.640 CLJ01 FAMILY 4 3.004 2.972 2.777 2.918 CLJ01 FAMILY 5 2.810 2.738 2.477 2.675 CLJ01 FAMILY 6 3.246 2.898 2.962 3.035 CLJ01 FAMILY 7 2.685 2.621 2.419 2.575 CLJ01 FAMILY 8 2.706 2.561 2.536 2.601 CLJ01 FAMILY 9 2.341 2.096 2.247 2.228 CLJ01 FAMILY 10 2.688 2.418 2.437 2.514 CLJ01 FAMILY 11 2.821 2.682 2.559 2.687 CLJ01 FAMILY 12 2.527 2.638 2.517 2.560 CLJ01 FAMILY 13 2.126 2.025 1.899 2.017 CLJ01 FAMILY 14 3.009 2.735 2.860 2.868 CLJ01 FAMILY 15 2.872 2.644 2.781 2.766 CLJ01 FAMILY 16 2.565 2.272 2.345 2.394 CLJ01 FAMILY 17 2.625 2.593 2.522 2.580 CLJ01 FAMILY 18 2.317 2.275 2.264 2.286 CLJ01 FAMILY 19 2.740 2.869 2.841 2.817 CLJ01 FAMILY 20 2.753 2.643 2.659 2.685 CLJ01 RRS 2.404 2.452 2.296 2.384 CLJOI Evangeline 2.308 2.140 2.268 2.239 CLJ01 ST. Landry 1.066 1.059 1.224 1.116 CLJ01 RRS 1.863 1.830 1.759 1.817 (2016 sample) CLPY 1090 0.414 0.450 0.476 0.447 CLPY 1096 0.173 0.188 0.169 0.177 CLPY 1097 0.114 0.095 0.125 0.111 CLPY 1101 1.146 1.044 1.017 1.069 CLPY 1102 0.926 0.961 1.053 0.980 CLPY 1103 1.965 1.906 1.940 1.937 CLPY 1104 2.652 2.552 2.560 2.588 CLPY 1107 2.668 2.587 2.691 2.649 CLPY 1112 1.488 1.605 1.420 1.504 KDML105 (2016 2.069 1.892 1.824 1.928 sample) (check)

TABLE 22 2AP aroma values of CLJ01, Jazzman2, Della2, CL111, CL151 and CL153 from 2017 Commercial Advanced multiple locations Analysis TEST REP 1 REP 2 REP 3 AVERAGE NOV. 27, CLJ01 (CLPY check) 1.049 1.124 1.101 1.091 2017 CLJ01 RRS 1.378 1.328 1.289 1.331 CLJ01 Acadia 1.257 1.162 1.232 1.217 CLJ01 Lake Arthur 1.613 1.522 1.949 1.695 Jazzman2 RRS 1.932 1.956 2.090 1.993 Jazzman2 Acadia 1.669 1.577 1.565 1.604 Jazzman2 Lake Arthur 2.433 2.355 2.370 2.386 Della2 RRS 1.490 1.480 1.564 1.511 Della2 Acadia 1.233 1.292 1.357 1.294 Della2 Lake Arthur n/a 1.446 1.483 1.464 CL111 RRS 0 0 0 0 CL111 Acadia 0 0 0 0 CL111 Lake Arthur 0 0 0 0 CL151 RRS 0 0 0 0 CL151 Acadia 0 0 0 0 CL151 Lake Arthur 0 0 0 0 CL153 RRS 0 0 0 0 CL153 Acadia 0 0 0 0 CL153 Lake Arthur 0 0 0 0

The variety is resistant to imidazolinone herbicides. The herbicide resistance profile is essentially the same as that of ‘CL161’ through common ancestry. The herbicide tolerance allows ‘CLJ01,’ its hybrids, and derived varieties to be used with Clearfield™ rice technology and herbicides, including among others the herbicides imazethapyr and imazamox, for the selective control of weeds, including red rice. See generally U.S. Pat. No. 6,943,280.

Herbicide tolerance and susceptibility characteristics: The variety is tolerant to some herbicides, and susceptible to some herbicides, that normally inhibit the growth of rice plants. Among others, the herbicide tolerance and susceptibility characteristics of ‘CLJ01’ include or are expected to include the following. These characteristics are in some cases based on actual observations to date, and in other cases reflect assumptions based on common ancestry with ‘CL161’:

‘CLJ01’ expresses a mutant acetohydroxyacid synthase whose enzymatic activity is directly resistant to normally-inhibitory levels of a herbicidally-effective imidazolinone;

‘CLJ01’ is resistant to each of the following imidazolinone herbicides, at levels of the imidazolinone herbicides that would normally inhibit the growth of a rice plant: imazethapyr, imazapic, imazaquin, imazamox, and imazapyr;

‘CLJ01’ is resistant to each of the following sulfonylurea herbicides, at levels of the sulfonylurea herbicides that would normally inhibit the growth of a rice plant: nicosulfuron, metsulfuron methyl, thifensulfuron methyl, and tribenuron methyl;

‘CLJ01’ is sensitive to each of the following sulfonylurea herbicides, at levels of the sulfonylurea herbicides that would normally inhibit the growth of a rice plant: sulfometuron methyl, chlorimuron ethyl, and rimsulfuron.

This invention is also directed to methods for producing a rice plant by crossing a first parent rice plant with a second parent rice plant to produce an F₁ hybrid, wherein the first or second rice plant (i.e., the male parent or the female parent) is a rice plant from the line ‘CLJ01.’ The F₁ hybrid preferably displays both the herbicide tolerance phenotype and the aromatic phenotype of ‘CLJ01.’ Further, both the first and second parent rice plants may be from the cultivar ‘CLJ01.’ Breeding methods that employ the cultivar ‘CLJ01’ are also part of this invention, including crossing, selfing, backcrossing, hybrid breeding, crossing to populations, the other breeding methods discussed in this specification, and other breeding methods otherwise known to those of skill in the art. Any plants produced using cultivar ‘CLJ01’ as a parent or ancestor by any of these breeding methods are within the scope of this invention, particularly when those plants display the herbicide tolerance and aromatic phenotypes of ‘CLJ01.’ The other parents or other lines used in such breeding programs may be any of the wide number of rice varieties, cultivars, populations, experimental lines, and other sources of rice germplasm known to the art.

For example, this invention includes methods for producing a first-generation hybrid rice plant by crossing a first parent rice plant with a second parent rice plant, wherein either the first or second parent rice plant (i.e., either the male parent or the female parent) is ‘CLJ01.’ Further, this invention is also directed to methods for producing a hybrid rice line derived from ‘CLJ01’ by crossing ‘CLJ01’ with a second rice plant, and growing the F₁ progeny seed. The crossing and growing steps may be repeated any number of times. Breeding methods using the rice line ‘CLJ01’ are considered part of this invention, not only backcrossing and hybrid production, but also selfing, crosses to populations, and other breeding methods known in the art. It is preferred that, at each step in the breeding process, there should be selection to maintain the herbicide tolerance trait.

Optionally, either of the parents in such a cross, ‘CLJ01’ or the other parent, may be produced in male-sterile form, using techniques otherwise known in the art.

In one embodiment, a rice plant produced using cultivar ‘CLJ01’ as a parent or ancestor exhibits tolerance to applications of one or more classes of herbicides. Classes of herbicides include, but are not limited to, acetohydroxyacid synthase (AHAS) inhibitors; bleaching herbicides such as hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors or phytoene desaturase (PDS) inhibitors; enolpyruvyl shikimate 3-phosphate synthase (EPSPS) inhibitors such as glyphosate; glutamine synthetase (GS) inhibitors such as glufosinate; auxinic herbicides, e.g., dicamba; lipid biosynthesis inhibitors such as ACCase inhibitors; or oxynil (i.e. bromoxynil or ioxynil) herbicides; protoporphyrinogen-IX oxidase (PPO) inhibitors other than saflufenacil (“other PPO inhibitors”) (e.g., acifluorfen, butafenacil, carfentrazone, flufenpyr-ethyl, fomesafen, flumiclorac, flumioxazin, lactofen, oxadiargyl, oxadiazon, oxyfluorfen, sulfentrazone); lipid biosynthesis inhibitors such as acetyl CoA carboxylase (ACCase) inhibitors; oxynil (i.e. bromoxynil or ioxynil) herbicides; ACCase-inhibitor(s); saflufenacil(s); p-hydroxyphenylpyruvate dioxygenase (4-HPPD) inhibitors; amide(s), e.g., propanil; and the like. AHAS-inhibitor herbicides include, e.g., imidazolinone herbicides, one or more sulfonylurea (SU) herbicides selected from the group consisting of amidosulfuron, flupyrsulfuron, foramsulfuron, imazosulfuron, iodosulfuron, mesosulfuron, nicosulfuron, thifensulfuron, and tribenuron, agronomically acceptable salts and esters thereof, and combinations thereof. ACCase inhibitor herbicides include, e.g., “dims” (e.g., cycloxydim, sethoxydim, clethodim, or tepraloxydim), “fops” (e.g., clodinafop, diclofop, fluazifop, haloxyfop, or quizalofop), and “dens” (such as pinoxaden).

In some embodiments rice plants that are produced using cultivar ‘CLJ01’ as a parent or ancestor may be tolerant to ACCase inhibitors, such as the “dims” (e.g., cycloxydim, sethoxydim, clethodim, or tepraloxydim), the “fops” (e.g., clodinafop, diclofop, fluazifop, haloxyfop, or quizalofop), and the “dens” (such as pinoxaden); to auxinic herbicides, such as dicamba; to EPSPS inhibitors, such as glyphosate; to other PPO inhibitors; and to GS inhibitors, such as glufosinate.

In addition to these classes of inhibitors, rice plants that are produced using cultivar ‘CLJ01’ as a parent or ancestor may also be tolerant to herbicides having other modes of action, for example, chlorophyll/carotenoid pigment inhibitors, cell membrane disruptors, photosynthesis inhibitors, cell division inhibitors, root inhibitors, shoot inhibitors, and combinations thereof.

Such tolerance traits may be expressed, e.g., as mutant acetohydroxyacid synthase large subunit (AHASL) proteins, mutant ACCase proteins, mutant EPSPS proteins, or mutant glutamine synthetase proteins; or as a mutant native, inbred, or transgenic aryloxyalkanoate dioxygenase (AAD or DHT), haloarylnitrilase (BXN), 2,2-dichloropropionic acid dehalogenase (DEH), glyphosate-N-acetyltransferase (GAT), glyphosate decarboxylase (GDC), glyphosate oxidoreductase (GOX), glutathione-S-transferase (GST), phosphinothricin acetyltransferase (PAT or bar), or cytochrome P450 (CYP450) protein having herbicide-degrading activity.

The rice plants hereof can also optionally be “stacked” with other traits including, but not limited to, pesticidal traits such as Bt Cry and other proteins having pesticidal activity toward coleopteran, lepidopteran, nematode, or other pests; nutritional or nutraceutical traits such as modified oil content or oil profile traits, high protein or high amino acid concentration traits, and other trait types known in the art.

Furthermore, in another embodiment, rice plants are generated, e.g. by the use of recombinant DNA techniques, breeding, or otherwise by selection for desired traits, plants that are able to synthesize one or more proteins to improve their productivity, oil content, tolerance to drought, salinity or other growth-limiting environmental factors, or tolerance to arthropod, fungal, bacterial, or viral pests or pathogens of rice plants.

Furthermore, in other embodiments, rice plants are generated, e.g. by the use of recombinant DNA techniques, breeding, or otherwise by selection for desired traits to contain a modified amount of one or more substances or to contain one or more new substances, for example, to improve human or animal nutrition, e.g. health-promoting long-chain omega-3 fatty acids or unsaturated omega-9 fatty acids. (Cf. Nexera® canola, Dow Agro Sciences, Canada).

Furthermore, in some embodiments, rice plants are generated, e.g. by the use of recombinant DNA techniques, breeding, or otherwise by selection for desired traits to contain increased amounts of vitamins, minerals, or improved profiles of nutraceutical compounds.

In one embodiment, rice plants are produced using cultivar ‘CLJ01’ as a parent or higher-generation ancestor so that the new rice plants, relative to a wild-type rice plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: glucosinolates (e.g., glucoraphanin (4-methylsulfinylbutyl-glucosinolate), sulforaphane, 3-indolylmethyl-glucosinolate (glucobrassicin), or 1-methoxy-3-indolylmethyl-glucosinolate (neoglucobrassicin)); phenolics (e.g., flavonoids (e.g., quercetin, kaempferol), hydroxycinnamoyl derivatives (e.g., 1,2,2′-trisinapoylgentiobiose, 1,2-diferuloylgentiobiose, 1,2′-disinapoyl-2-feruloylgentiobiose, or 3-O-caffeoyl-quinic (neochlorogenic acid)); and vitamins and minerals (e.g., vitamin C, vitamin E, carotene, folic acid, niacin, riboflavin, thiamine, calcium, iron, magnesium, potassium, selenium, and zinc).

In another embodiment, rice plants are produced using cultivar ‘CLJ01’ as a parent or higher-generation ancestor so that the new rice plants, relative to a wild-type rice plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: progoitrin; isothiocyanates; indoles (products of glucosinolate hydrolysis); glutathione; carotenoids such as beta-carotene, lycopene, and the xanthophyll carotenoids such as lutein and zeaxanthin; phenolics comprising the flavonoids such as the flavonols (e.g. quercetin, rutin), the flavins/tannins (such as the procyanidins comprising coumarin, proanthocyanidins, catechins, and anthocyanins); flavones; phytoestrogens such as coumestans; lignans; resveratrol; isoflavones e.g. genistein, daidzein, and glycitein; resorcyclic acid lactones; organosulfur compounds; phytosterols; terpenoids such as carnosol, rosmarinic acid, glycyrrhizin and saponins; chlorophyll; chlorphyllin, sugars, anthocyanins, and vanilla.

Herbicides

Herbicidal compositions that may be used in conjunction with the invention include herbicidally active ingredients (A.I.), and their agronomically acceptable salts and esters.

The herbicidal compositions can be applied in any agronomically acceptable format. For example, they can be formulated as ready-to-spray aqueous solutions, powders, or suspensions; as concentrated or highly concentrated aqueous, oily or other solutions, suspensions or dispersions; as emulsions, oil dispersions, pastes, dusts, granules, or other broadcastable formats. The herbicidal compositions can be applied by any method known in the art, including, for example, spraying, atomizing, dusting, spreading, watering, seed treatment, or co-planting in admixture with the seed. The particular formulation used depends on the intended purpose; in any case, it should ensure a uniform (or approximately uniform) distribution of the A.I. or A.I.s. A herbicidal composition can be selected according to the tolerances of a particular plant, and the plant can be selected from among those having a single tolerance trait, or stacked tolerance traits.

In some embodiments, where the A.I. includes an AHAS inhibitor, the AHAS inhibitor may be selected from: (1) the imidazolinones, e.g. imazamox, imazethapyr, imazapyr, imazapic, imazaquin, and imazamethabenz; preferably imazamox, imazethapyr, imazapyr, or imazapic; (2) the sulfonylureas, e.g. amidosulfuron, azimsulfuron, bensulfuron, cinosulfuron, ethoxysulfuron, flupyrsulfuron, foramsulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron, nicosulfuron, thifensulfuron, and tribenuron; (3) the pyrimidinyloxy [thio] benzoates, e.g. including the pyrimidinyloxybenzoates (e.g., bispyribac, pyriminobac, and pyribenzoxim) and the pyrimidinylthiobenzoates (e.g., pyrithiobac and pyriftalid); and (4) the sulfonamides, e.g. including the sulfonylaminocarbonyltriazolinones (e.g., flucarbazone and propoxycarbazone) and the triazolopyrimidines (e.g., cloransulam, diclosulam, florasulam, flumetsulam, metosulam, and penoxsulam). The agronomically acceptable salts and esters of the foregoing are also included, as are combinations thereof.

In embodiments in which the A.I. includes an ACCase inhibitor, the ACCase inhibitor may for example be selected from: aryloxyphenoxypropionate (FOP) herbicides, cyclohexanedione (DIM) herbicides, and phenylpyrazoline (DEN) herbicides, and their agronomically acceptable salts and esters. Examples include: the DIMs, e.g., cycloxydim, sethoxydim, clethodim, or tepraloxydim; the FOPs, e.g., clodinafop, diclofop, fluazifop, haloxyfop, or quizalofop; and the DENs, e.g., pinoxaden, Preferred esters of quizalofop or quizalofop-P include the ethyl and tefuryl esters; and preferred esters of haloxyfop or haloxyfop-P include the methyl and etotyl esters.

The agronomically acceptable salts and esters of the foregoing are also included, as are combinations thereof.

Examples of herbicides that are ACCase inhibitors include, but are not limited to, cyclohexanedione herbicides (DIMs, also referred to as: cyclohexene oxime cyclohexanedione oxime; and CHD), aryloxyphenoxy propionate herbicides (also referred to as aryloxyphenoxy propanoate; aryloxyphenoxyalkanoate; oxyphenoxy; APP; AOPP; APA; APPA; FOP), and phenylpyrazole herbicides (also known as DENs; and sometimes referred to under the more general class of phenylpyrazoles such as pinoxaden (e.g., herbicides sold under the trade names Axial and Traxos)). In some methods of controlling weeds or growing herbicide-tolerant plants, at least one herbicide is selected from the group consisting of sethoxydim, cycloxydim, tepraloxydim, haloxyfop, haloxyfop-P or a derivative of one of these herbicides. Table C lists examples of herbicides that interfere with ACCase activity.

TABLE C Examples of ACCase inhibitors. ACCase Inhibitor Class Company Examples of Synonyms and Trade Names alloxydim DIM BASF Fervin, Kusagard, NP-48Na, BAS 9021H, Carbodimedon, Zizalon butroxydim DIM Syngenta Falcon, ICI-A0500, Butroxydim clethodim DIM Valent Select, Prism, Centurion, RE-45601, Motsa Clodinafop- FOP Syngenta Discover, Topik, CGA 184 927 propargyl clofop FOP Fenofibric Acid, Alopex cloproxydim FOP chlorazifop FOP cycloxydim DIM BASF Focus, Laser, Stratos, BAS 517H cyhalofop-butyl FOP Dow Clincher, XDE 537, DEH 112, Barnstorm diclofop-methyl FOP Bayer Hoegrass, Hoelon, Illoxan, HOE 23408, Dichlorfop, Illoxan fenoxaprop-P- FOP Bayer Super Whip, Option Super, Exel Super, HOE-46360, ethyl Aclaim, Puma S, Fusion fenthiaprop FOP Taifun; Joker fluazifop-P- FOP Syngenta Fusilade, Fusilade 2000, Fusilade DX, ICI-A 0009, butyl ICI-A 0005, SL-236, IH-773B, TF-1169, Fusion haloxyfop-etotyl FOP Dow Gallant, DOWCO 453EE haloxyfop-methyl FOP Dow Verdict, DOWCO 453ME haloxyfop-P- FOP Dow Edge, DE 535 methyl isoxapyrifop FOP Metamifop FOP Dongbu NA pinoxaden DEN Syngenta Axial profoxydim DIM BASF Aura, Tetris, BAS 625H, Clefoxydim propaquizafop FOP Syngenta Agil, Shogun, Ro 17-3664, Correct quizalofop-P- FOP DuPont Assure, Assure II, DPX-Y6202-3, Targa Super, NC-302, ethyl Quizafop quizalofop-P- FOP Uniroyal Pantera, UBI C4874 tefuryl sethoxydim DIM BASF Poast, Poast Plus, NABU, Fervinal, NP-55, Sertin, BAS 562H, Cyethoxydim, Rezult tepraloxydim DIM BASF BAS 620H, Aramo, Caloxydim tralkoxydim DIM Syngenta Achieve, Splendor, ICI-A0604, Tralkoxydime, Tralkoxidym trifop FOP

Examples of herbicides that are auxinic herbicides include, but are not limited to, those shown in Table D.

TABLE D Examples of Auxinic herbicides. Classification of Auxinic Herbicides (HRAC Group ‘O’; WSSA Group ‘4’) Subgroup Member Compound Phenoxy- Clomeprop carboxylic- cloprop (“3-CPA”) acid Subgroup 4-chlorophenoxyacetic acid (“4-CPA”) 2-(4-chlorophenoxy)propionic acid (“4-CPP”) 2,4-dichlorophenoxy acetic acid (“2,4-D”) (3,4-dichlorophenoxy)acetic acid (“3,4-DA”) 4-(2,4-dichlorophenoxy)butyric acid (“2,4-DB”) 2-(3,4-dichlorophenoxy)propionic acid (“3,4-DP”) tris[2-(2,4-dichlorophenoxy)ethyl]phosphite (“2,4-DEP”) dichlorprop (“2,4-DP”) 2,4,5-trichlorophenoxyacetic acid (“2,4,5-T”) fenoprop (“2,4,5-TP”) 2-(4-chloro-2-methylphenoxy)acetic acid (“MCPA”) 4-(4-chloro-2-methylphenoxy)butyric acid (“MCPB”) mecoprop (“MCPP”) Benzoic acid Chloramben Subgroup Dicamba Tricamba 2,3,6-trichlorobenzoic acid (“TBA”) Pyridine Aminopyralid carboxylic Clopyralid acid Subgroup Fluroxypyr Picloram Triclopyr Quinoline Quinclorac carboxylic Quinmerac acid Subgroup Other Benazolin Subgroup

Optional A.I.s of other types include, but are not limited to agronomically-acceptable fungicides such as strobilurins, e.g., pyraclostrobin, alone or in combination with, e.g., boscalid, epiconazole, metaconazole, tebuconazole, kresoxim-methyl, and the like; insecticides, nematicides, lepidoptericides, coleoptericides, or molluscicides (e.g., malathion, pyrethrins/pyrethrum, carbaryl, spinosad, permethrin, bifenthrin, and esfenvalerate).

In one embodiment, a saflufenacil A.I. is, e.g.: 2-chloro-5-[3,6-dihydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)-1-(2H)-pyrimidinyl]-4-fluoro-N-[[methyl(1-methylethyl) amino] sulfonyl] benzamide (CAS: N′-{2-chloro-4-fluoro-5-[1,2,3,6-tetrahydro-3-methyl-2,6-dioxo-4-(trifluoromethyl)pyrimidin-1-yl] benzoyl}-N-isopropyl-N-methylsulfamide; Reg. No.: 372137-35-4); BAS-H800).

As used herein, unless context clearly indicates others, a reference to a named compound, (e.g., “saflufenacil”) should be understood to include not only the specified compound itself, but also the compound's various salts and esters.

The herbicidal compositions can also comprise auxiliary ingredients that are customary for the formulation of crop protection agents. Examples of auxiliaries customary for the formulation of crop protection agents include inert auxiliaries, solid carriers, surfactants (such as dispersants, protective colloids, emulsifiers, wetting agents, and tackifiers), organic and inorganic thickeners, penetrants (such as penetration-enhancing organosilicone surfactants or acidic sulfate chelates, e.g., CT-301™ available from Cheltec, Inc.), safeners, bactericides, antifreeze agents, antifoams, colorants, and adhesives. Formulations of the herbicide compositions useful herein can be prepared according to any method useful for that purpose in the art.

Examples of thickeners (i.e. compounds that modify flow properties, e.g. high viscosity in a state of rest and low viscosity in motion) include polysaccharides, such as xanthan gum (Kelzan® from Kelco), Rhodopol® 23 (Rhone Poulenc) or Veegum® (from R. T. Vanderbilt), and also various organic and inorganic sheet minerals, such as Attaclay® (from Engelhard).

Examples of antifoaming agents include silicone emulsions (for example, Silikon® SRE, Wacker or Rhodorsil® from Rhodia), long-chain alcohols, fatty acids, salts of fatty acids, organofluorine compounds, and mixtures thereof.

Bactericides can optionally be added for stabilizing the aqueous herbicidal formulations. Examples include bactericides based on diclorophen and benzyl alcohol hemiformal (Proxel® from ICI, Acticide® RS from Thor Chemie, or Kathon® MK from Rohm & Haas), or isothiazolinone derivatives, such as alkylisothiazolinones and benzisothiazolinones (Acticide MBS from Thor Chemie).

Examples of antifreeze agents include ethylene glycol, propylene glycol, urea, and glycerol.

Examples of colorants include members of colorant classes such as the sparingly water-soluble pigments and the water-soluble dyes. Some examples include the dyes known under the names Rhodamin B, C.I. Pigment Red 112, C. I. Solvent Red 1, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1, pigment red 57:1, pigment red 53:1, pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, and basic red 108.

Examples of adhesives include polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol, and tylose.

Suitable inert auxiliaries include, for example, the following: mineral oil fractions of medium to high boiling point, such as kerosene and diesel oil; coal tar oils; oils of vegetable or animal origin; aliphatic, cyclic and aromatic hydrocarbons, for example paraffins, tetrahydronaphthalene, alkylated naphthalenes and their derivatives, and alkylated benzenes and their derivatives; alcohols such as methanol, ethanol, propanol, butanol and cyclohexanol; ketones such as cyclohexanone; strongly polar solvents, for example amines such as N-methylpyrrolidone; and water; as well as mixtures thereof.

Suitable carriers include liquid and solid carriers.

Liquid carriers include e.g. non-aqueous solvents such as cyclic and aromatic hydrocarbons, e.g. paraffins, tetrahydronaphthalene, alkylated naphthalenes and their derivatives, and alkylated benzenes and their derivatives; alcohols such as methanol, ethanol, propanol, butanol and cyclohexanol; ketones such as cyclohexanone; strongly polar solvents, e.g. amines such as N-methylpyrrolidone; and water; as well as mixtures thereof.

Solid carriers include e.g. mineral earths such as silicas, silica gels, silicates, talc, kaolin, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate, and magnesium oxide; ground synthetic materials; fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, and ureas; and products of vegetable origin, such as cereal meal, tree bark meal, wood meal, nutshell meal, and cellulose powders; and mixtures thereof.

Suitable surfactants (e.g., adjuvants, wetting agents, tackifiers, dispersants, or emulsifiers) include the alkali metal salts, alkaline earth metal salts, and ammonium salts of aromatic sulfonic acids, for example lignosulfonic acids (e.g. Borrespers-types, Borregaard), phenolsulfonic acids, naphthalenesulfonic acids (Morwet types, Akzo Nobel) and dibutylnaphthalenesulfonic acid (Nekal types, BASF AG); and salts of fatty acids, alkyl- and alkylarylsulfonates, alkyl sulfates, lauryl ether sulfates and fatty alcohol sulfates; and salts of sulfated hexa-, hepta- and octadecanols; fatty alcohol glycol ethers, condensates of sulfonated naphthalene and its derivatives with formaldehyde, condensates of naphthalene or of the naphthalenesulfonic acids with phenol and formaldehyde, polyoxyethylene octylphenol ether, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ether, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol/ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers or polyoxypropylene alkyl ethers, lauryl alcohol polyglycol ether acetate, sorbitol esters; lignosulfite waste liquors; and proteins, denatured proteins, polysaccharides (e.g. methylcellulose), hydrophobically modified starches, polyvinyl alcohol (Mowiol types, Clariant), polycarboxylates (BASF AG, Sokalan types), polyalkoxylates, polyvinylamine (BASF AG, Lupamine types), polyethyleneimine (BASF AG, Lupasol types), polyvinylpyrrolidone, and copolymers thereof; and mixtures thereof.

Powders, materials for broadcasting and dusts can be prepared by mixing or concomitant grinding of the A.I.s together with a solid carrier.

Granules, for example coated granules, impregnated granules and homogeneous granules, can be prepared by binding the A.I.s to solid carriers.

Aqueous-use forms can be prepared from emulsion concentrates, suspensions, pastes, wettable powders or water-dispersible granules by adding water.

To prepare emulsions, pastes, or oil dispersions, the herbicidal compositions can be homogenized in water by means of a wetting agent, tackifier, dispersant or emulsifier. Alternatively, it is also possible to prepare concentrates comprising active compound, wetting agent, tackifier, dispersant or emulsifier and, if desired, solvent or oil, preferably suitable for dilution or dispersion with water.

The concentration of the herbicide(s) present in the herbicidal composition can be varied within wide ranges. In general, the formulations comprise approximately from 0.001% to 98% by weight, preferably 0.01 to 95% by weight of at least one active ingredient. In some embodiments, the A.I.s are employed in a purity of from 90% to 100%, preferably 95% to 100% (as measured, e.g., by NMR or IR spectra).

In some formulations, the herbicides are suspended, emulsified, or dissolved. The formulations may be in the form of aqueous solutions, powders, suspensions, or highly-concentrated aqueous, oily or other suspensions or dispersions, aqueous emulsions, aqueous microemulsions, aqueous suspo-emulsions, oil dispersions, pastes, dusts, materials for spreading, or granules.

Herbicides or herbicidal compositions can be applied pre-emergence, post-emergence, or pre-planting, or together with the seed. It is also possible to apply the herbicidal composition or active compounds by planting seed pretreated with the herbicidal compositions or active compounds.

In a further embodiment, the herbicides or herbicidal compositions can be applied by treating seed. The treatment of seeds comprises any of the procedures known in the art (e.g., seed dressing, seed coating, seed dusting, seed soaking, seed film coating, seed multilayer coating, seed encrusting, seed dripping, and seed pelleting). The herbicidal compositions can be applied diluted or undiluted.

It may be beneficial in some embodiments to apply the herbicides alone or in combination with other herbicides, or in the form of a mixture with other crop protection agents, for example together with agents for controlling pests or phytopathogenic fungi or bacteria. Also of interest is miscibility with mineral salt solutions, which are employed for treating nutritional and trace element deficiencies. Other additives such as non-phytotoxic oils and oil concentrates can also be added.

Moreover, it may be useful to apply the herbicides in combination with safeners. Safeners are compounds that prevent or reduce herbicide-induced injury to useful plants without having a major effect on the intended herbicidal action of the herbicides. They can be applied either before sowing (e.g. on seed treatments, shoots or seedlings) or in the pre-emergence application or post-emergence application of the crop plant. The safeners and the herbicides can be applied simultaneously or in succession.

Safeners include e.g. (quinolin-8-oxy)acetic acids, 1-phenyl-5-haloalkyl-1H-1,2,4-triazol-3-carboxy c acids, 1-phenyl-4,5-dihydro-5-alkyl-1H-pyrazol-3,5-dicarboxylic acids, 4,5-dihydro-5,5-diaryl-3 isoxazol carboxylic acids, dichloroacetamides, alpha-oximinophenylacetonitriles, acetophenonoximes, 4,6-dihalo-2-phenylpyrimidines, N-[[4-(aminocarbonyl)phenyl]sulfonyl]-2-benzoic amides, 1,8-naphthalic anhydride, 2-halo-4-(haloalkyl)-5-thiazol carboxylic acids, benoxacor, cloquintocet, cyometrinil, cyprosulfamide, dichlormid, dicyclonon, dietholate, fenchlorazole, fenclorim, flurazole, fluxofenim, furilazole, isoxadifen, mefenpyr, mephenate, naphthalic anhydride, oxabetrinil, 4-(dichloroacetyl)-1-oxa-4-azaspiro[4.5]decane (MON4660, CAS 71526-07-3) and 2,2,5-trimethyl-3-(dichloroacetyl)-1,3-oxazolidine (R-29148, CAS 52836-31-4), phosphorthiolates, and N-alkyl-O-phenyl-carbamates and their agriculturally-acceptable salts and their agriculturally-acceptable derivatives such amides, esters, and thioesters.

Those skilled in the art will recognize that some compounds used as herbicides, safeners, etc. are capable of forming geometric isomers, for example E/Z isomers, enantiomers, diastereomers, or other stereoisomers. In general, it is possible to use either pure isomers or mixtures of isomers. For example, some of the aryloxyphenoxy propionate herbicides are chiral, and some of them are commonly used in enantiomerically enriched or enantiopure form, e.g. clodinafop, cyhalofop, fenoxaprop-P, fluazifop-P, haloxyfop-P, metamifop, propaquizafop or quizalofop-P. As a further example, glufosinate may be used in enantiomerically enriched or enantiopure form, also known as glufosinate-P. Alternatively, the compounds may be used in racemic mixtures or other mixtures of geometric isomers.

Controlling Weeds

Rice plants of the invention can be used in conjunction with herbicide(s) to which they are tolerant. Herbicides can be applied to the rice plants of the invention using any techniques known to those skilled in the art. Herbicides can be applied at any point in the rice plant cultivation process. For example, herbicides can be applied pre-planting, at planting, pre-emergence, post-emergence or combinations thereof. Herbicides may be applied to seeds and dried to form a layer on the seeds.

In some embodiments, seeds are treated with a safener, followed by a post-emergence application of herbicide(s). In one embodiment, the post-emergence application of herbicide(s) occurs about 7 to 10 days following planting of safener-treated seeds. In some embodiments, the safener is cloquintocet, dichlormid, fluxofenim, or combinations thereof.

In other aspects, the present invention provides a method for controlling weeds at a locus for growth of a rice plant or plant part thereof, the method comprising applying a composition comprising herbicide(s) to the locus.

In some aspects, the present invention provides a method for controlling weeds at a locus for growth of a plant, the method comprising applying a herbicide composition to the locus; wherein said locus is: (a) a locus that contains a rice plant or seed capable of producing a rice plant; or (b) a locus that will contain the rice plant or the seed after the herbicide composition is applied.

Following are non-limiting examples of various rice culturing methods, including the application of herbicide(s).

In the post-flood, post-emergence (transplanted) method, rice is grown to about the 2-4 leaf stage away from the field. The field is flooded and tilled (puddled) until a blend of mud is achieved. The rice plants are then transplanted into the mud. Herbicide application typically takes place before or after flooding.

In the post-flood, post-emergence (water-seeded) method, rice is soaked for about 24 hours or more, and then is sown into the surface of a shallow flooded field. Herbicide application is typically made after weed germination.

In the pre-flood, post-emergence, direct-seeded (broadcast or drilled) method, rice is broadcast or planted with a planter under the soil surface. The field may be flushed (watered) to promote rice growth. The field is flooded about a week or more after planting as the plants germinate. Herbicide application takes place typically before the flood, but after emergence of the rice plants.

In the pre-flood, post-emergence (Southeast Asia style) method, rice is soaked for about 24 hours or more. The field is puddled to the right consistency and drained. The pre-germinated seeds are then broadcast to the surface of the soil. Flooding takes place as the rice develops. Herbicide application normally takes place before the flooding, but after the emergence of the rice plants.

In the pre-emergence or delayed pre-emergence method, seeds are planted, usually with a planter. Herbicide is applied before emergence of the rice or weeds.

Herbicide compositions can be applied, e.g., as foliar treatments, soil treatments, seed treatments, or soil drenches. Application can be made, e.g., by spraying, dusting, broadcasting, or any other mode known in the art.

In one embodiment, herbicides can be used to control the growth of weeds in the vicinity of the rice plants of the invention. A herbicide to which the rice plant of the invention is tolerant can be applied to the plot at a concentration sufficient to kill or inhibit the growth of weeds. Concentrations of herbicide sufficient to kill or inhibit the growth of weeds are known in the art for typical circumstances, and generally depend on the particulars of the herbicide, the weeds being controlled, the weather, the soil type, the degree of maturity of the weeds, and the like.

In another embodiment, the present invention provides a method for controlling weeds in the vicinity of rice plants. The method comprises applying an effective amount of herbicide(s) to the weeds and to the rice plant, wherein the rice plant has increased tolerance to the herbicide(s) when compared to a wild-type rice plant. An “effective amount” of herbicide is an amount that is sufficient to kill or inhibit the growth of particular weeds. What constitutes an “effective amount” depends on the particulars of the herbicide, the weeds being controlled, the weather, the soil type, the degree of maturity of the weeds, and the like; such “effective amounts” for typical circumstances are well known in the art.

In another aspect, herbicide(s) can be used as a seed treatment. In some embodiments, an effective concentration or an effective amount of herbicide(s), or a composition comprising an effective concentration or an effective amount of herbicide(s) can be applied directly to the seeds prior to or during the sowing of the seeds. Seed treatment formulations may additionally comprise binders, and optionally colorants as well.

Binders can be added to improve the adhesion of the active materials onto the seeds after treatment. Suitable binders include, e.g., block copolymers, EO/PO surfactants, polyvinylalcohols, polyvinylpyrrolidones, polyacrylates, polymethacrylates, polybutenes, polyisobutylenes, polystyrene, polyethyleneamines, polyethyleneamides, polyethyleneimines (e.g., Lupasol®, Polymin®), polyethers, polyurethanes, polyvinylacetate, tylose, and copolymers derived from these polymers.

The term “seed treatment” includes all suitable seed treatment techniques known in the art, including seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting. Alternatively, or in addition, soil may be treated by applying a formulation containing the herbicide (e.g., a granular formulation), for example with a seed drill, with optionally one or more solid or liquid, agriculturally acceptable carriers, and optionally with one or more agriculturally acceptable surfactants.

The present invention also comprises seeds coated with or containing a seed treatment formulation comprising herbicide(s) or other compounds. The term “coated with or containing” generally signifies that the active ingredient is for the most part on the surface of the seed at the time of application, although a greater or lesser part of the ingredient may penetrate into the seed, depending on the method of application. When the seed is planted, it may absorb the active ingredient.

In some embodiments, the seed treatment with herbicide(s) or with a formulation comprising the herbicide(s) is applied by spraying or dusting the seeds, or otherwise treating the seeds, before the seeds are sown. Alternatively, the seed treatment can comprise any one or more of the agriculturally acceptable herbicides, fungicides, insecticides, or nematicides, or combination thereof.

In other aspects, the present invention provides a method for combating undesired vegetation or controlling weeds, comprising contacting seeds of the rice plants with herbicide(s) before sowing, or after pre-germination, or both. The method can further comprise sowing the seeds, for example, in soil in a field or in a potting medium in a greenhouse. The method finds particular use in combating undesired vegetation or controlling weeds in the immediate vicinity of the seed. The control of undesired vegetation is understood as the killing of weeds, or otherwise retarding or inhibiting the normal growth of weeds. “Weeds,” in the broadest sense, should be understood as including all plants that grow in locations where they are undesired.

The weeds that may be treated include, for example, dicotyledonous and monocotyledonous weeds. Monocotyledonous weeds include, but are not limited to, weeds of the genera: Echinochloa, Setaria, Panicum, Digitaria, Phleum, Poa, Festuca, Eleusine, Brachiaria, Lolium, Bromus, Avena, Oryza, Cyperus, Sorghum, Agropyron, Cynodon, Monochoria, Fimbristyslis, Sagittaria, Eleocharis, Scirpus, Paspalum, Ischaemum, Sphenoclea, Dactyloctenium, Agrostis, Alopecurus, Anomalocaris, and Apera.

Dicotyledonous weeds include, but are not limited to, weeds of the genera: Sinapis, Lepidium, Galium, Stellaria, Matricaria, Anthemis, Galinsoga, Chenopodium, Urtica, Senecio, Amaranthus, Portulaca, Xanthium, Convolvulus, Ipomoea, Polygonum, Sesbania, Ambrosia, Cirsium, Carduus, Sonchus, Solanum, Rorippa, Rotala, Lindernia, Lamium, Veronica, Abutilon, Emex, Datura, Viola, Galeopsis, Papaver, Centaurea, Trifolium, Ranunculus, Wiwaxia, and Taraxacum.

Examples of red/weedy rice include, but are not limited to, Oryza longistaminata, Oryza sativa L. var. sylvatica, Oryza latifolia, Oryza barthii A. Chev, Oryza punctata, and Oryza rufipogon.

Examples of Echinochloa spp. include, but are not limited to, Echinochloa colona, Echinochloa crusgalli, and Echinochloa oryzicola.

In addition, the weeds treated with the present invention can include, for example, crop plants that are growing in an undesired location.

In still further aspects, loci, plants, plant parts, or seeds are treated with an agronomically acceptable composition that does not contain an A.I. For example, the treatment may comprise one or more agronomically-acceptable carriers, diluents, excipients, plant growth regulators, and the like; or an adjuvant, such as a surfactant, a spreader, a sticker, a penetrant, a drift-control agent, a crop oil, an emulsifier, a compatibility agent, or combinations thereof.

In other aspects, the present invention provides a product prepared from the rice plants of the invention, for example, brown rice (e.g., cargo rice), broken rice (e.g., chits, brewer's rice), polished rice (e.g., milled rice), rice hulls (e.g., husks, chaff), rice bran, rice pollards, rice mill feed, rice flour, rice oil, oiled rice bran, de-oiled rice bran, arrak, rice wine, poultry litter, and animal feed.

Further Embodiments of the Invention

As used herein, the term “plant” includes plant cells, plant protoplasts, plant cells of tissue culture from which rice plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as pollen, flowers, embryos, ovules, seeds, pods, leaves, stems, roots, anthers and the like. Thus, another aspect of this invention is to provide for cells that, upon growth and differentiation, produce a cultivar having essentially all of the physiological and morphological characteristics of ‘CLJ01.’

Techniques for transforming with and expressing desired structural genes and cultured cells are known in the art. Also, as known in the art, rice may be transformed and regenerated such that whole plants containing and expressing desired genes under regulatory control are obtained. General descriptions of plant expression vectors and reporter genes and transformation protocols can be found, for example, in Gruber et al., “Vectors for Plant Transformation, in Methods in Plant Molecular Biology & Biotechnology” in Glich et al. (Eds. pp. 89-119, CRC Press, 1993). For example, expression vectors and gene cassettes with the GUS reporter are available from Clone Tech Laboratories, Inc. (Palo Alto, Calif.), and expression vectors and gene cassettes with luciferase reporter are available from Promega Corp. (Madison, Wis.). General methods of culturing plant tissues are provided, for example, by Maki et al., “Procedures for Introducing Foreign DNA into Plants” in Methods in Plant Molecular Biology & Biotechnology, Glich et al., (Eds. pp. 67-88 CRC Press, 1993); by Phillips et al., “Cell-Tissue Culture and In-Vitro Manipulation” in Corn & Corn Improvement, 3rd Edition; and by Sprague et al., (Eds. pp. 345-387) American Society of Agronomy Inc., 1988. Methods of introducing expression vectors into plant tissue include the direct infection or co-cultivation of plant cells with Agrobacterium tumefaciens, Horsch et al., Science, 227:1229 (1985). Descriptions of Agrobacterium vectors systems and methods for Agrobacterium-mediated gene transfer are provided by Gruber et al., supra.

Useful methods include but are not limited to expression vectors introduced into plant tissues using a direct gene transfer method such as microprojectile-mediated delivery, DNA injection, electroporation and the like. More preferably expression vectors are introduced into plant tissues using the microprojectile media delivery with biolistic device- or Agrobacterium-mediated transformation. Transformed plants obtained with the germplasm of ‘CLJ01’ are intended to be within the scope of this invention.

The present invention also provides rice plants regenerated from a tissue culture of the ‘CLJ01’ variety or hybrid plant. As is known in the art, tissue culture can be used for the in vitro regeneration of a rice plant. For example, see Chu, Q. R. et al. (1999) “Use of bridging parents with high anther culturability to improve plant regeneration and breeding value in rice,” Rice Biotechnology Quarterly, 38:25-26; Chu, Q. R. et al., “A novel plant regeneration medium for rice anther culture of Southern U.S. crosses,” Rice Biotechnology Quarterly, 35:15-16 (1998); Chu, Q. R. et al., “A novel basal medium for embryogenic callus induction of Southern US crosses,” Rice Biotechnology Quarterly, 32:19-20 (1997); and Oono, K., “Broadening the Genetic Variability By Tissue Culture Methods,” Jap. J Breed., 33 (Supp. 2), 306-307 (1983). Thus, another aspect of this invention is to provide cells that, upon growth and differentiation, produce rice plants having all, or essentially all, of the physiological and morphological characteristics of variety ‘CLJ01.’

Unless context clearly indicates otherwise, references in the specification and claims to ‘CLJ01’ should be understood also to include single gene conversions of ‘CLJ01’ with a gene encoding a trait such as, for example, male sterility, other sources of herbicide resistance, resistance for bacterial, fungal, or viral disease, insect resistance, male fertility, enhanced nutritional quality, industrial usage, yield stability and yield enhancement.

Duncan et al., Planta, 165:322-332 (1985) reflects that 97% of the plants cultured that produced callus were capable of plant regeneration. Subsequent experiments with both inbreds and hybrids produced 91% regenerable callus that produced plants. In a further study, Songstad et al., Plant Cell Reports, 7:262-265 (1988) reported several media additions that enhanced regenerability of callus of two inbred lines. Other published reports also indicate that “nontraditional” tissues are capable of producing somatic embryogenesis and plant regeneration. K. P. Rao et al., Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger et al., Plant Cell Reports, 6:345-347 (1987) reported somatic embryogenesis from the tissue cultures of corn leaf segments. These methods of obtaining plants are routinely used with a high rate of success.

Tissue culture of corn (maize) is described in European Patent Application No. 160,390. Corn tissue culture procedures, which may be adapted for use with rice, are also described in Green et al., “Plant Regeneration in Tissue Culture of Maize,” Maize for Biological Research (Plant Molecular Biology Association, Charlottesville, Va., pp. 367-372, 1982) and in Duncan et al., “The Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes,” 165 Planta, 322:332 (1985). Thus, another aspect of this invention is to provide cells that, upon growth and differentiation, produce rice plants having all, or essentially all, of the physiological and morphological characteristics of hybrid rice line ‘CLJ01.’ See T. P. Croughan et al., (Springer-Verlag, Berlin, 1991) Rice (Oryza sativa. L): Establishment of Callus Culture and the regeneration of Plants, in Biotechnology in Agriculture and Forestry (19-37).

With the advent of molecular biological techniques that allow the isolation and characterization of genes that encode specific protein products, it is now possible to routinely engineer plant genomes to incorporate and express foreign genes, or additional or modified versions of native, or endogenous, genes (perhaps driven by different promoters) in order to alter the traits of a plant in a specific manner. Such foreign, additional, and modified genes are herein referred to collectively as “transgenes.” In recent years, several methods for producing transgenic plants have been developed, and the present invention, in particular embodiments, also relates to transformed versions of ‘CLJ01.’

An expression vector is constructed that will function in plant cells. Such a vector comprises a DNA coding sequence that is under the control of or is operatively linked to a regulatory element (e.g., a promoter). The expression vector may contain one or more such operably linked coding sequence/regulatory element combinations. The vector(s) may be in the form of a plasmid or virus, and can be used alone or in combination with other plasmids or viruses to provide transformed rice plants.

Expression Vectors

Expression vectors commonly include at least one genetic “marker,” operably linked to a regulatory element (e.g., a promoter) that allows transformed cells containing the marker to be either recovered by negative selection, i.e., inhibiting growth of cells that do not contain the selectable marker gene, or by positive selection, i.e., screening for the product encoded by the genetic marker. Many commonly used selectable marker genes for plant transformation are known in the art, and include, for example, genes that code for enzymes that metabolically detoxify a selective chemical inhibitor such as an antibiotic or a herbicide, or genes that encode an altered target that is insensitive to such an inhibitor. Positive selection methods are also known in the art.

For example, a commonly used selectable marker gene for plant transformation is that for neomycin phosphotransferase II (nptII), isolated from transposon Tn5, whose expression confers resistance to kanamycin. See Fraley et al., Proc. Natl. Acad. Sci. U.S.A., 80:4803 (1983). Another commonly used selectable marker gene is the hygromycin phosphotransferase gene, which confers resistance to the antibiotic hygromycin. See Vanden Elzen et al., Plant Mol. Biol., 5:299 (1985).

Additional selectable marker genes of bacterial origin that confer resistance to one or more antibiotics include gentamycin acetyl transferase, streptomycin phosphotransferase, aminoglycoside-3’-adenyl transferase, and the bleomycin resistance determinant. Hayford et al., Plant Physiol., 86:1216 (1988), Jones et al., Mol. Gen. Genet., 210:86 (1987), Svab et al., Plant Mol. Biol., 14:197 (1990); Plant Mol. Biol., 7:171 (1986). Other selectable marker genes confer resistance to herbicides such as glyphosate, glufosinate, or broxynil. Comai et al., Nature, 317:741-744 (1985); Gordon-Kamm et al., Plant Cell, 2:603-618 (1990); and Stalker et al., Science, 242:419-423 (1988).

Selectable marker genes for plant transformation of non-bacterial origin include, for example, mouse dihydrofolate reductase, plant 5-enolpyruvylshikimate-3-phosphate synthase, and plant acetolactate synthase. Eichholtz et al., Somatic Cell Mol. Genet. 13:67 (1987); Shah et al., Science, 233:478 (1986); and Charest et al., Plant Cell Rep., 8:643 (1990).

Another class of marker genes for plant transformation employs screening of presumptively transformed plant cells, rather than selection for resistance to a toxic substance such as an antibiotic. These marker genes are particularly useful to quantify or visualize the spatial pattern of expression of a gene in specific tissues, and are frequently referred to as reporter genes because they may be fused to the target gene or regulatory sequence. Commonly used reporter genes include glucuronidase (GUS), galactosidase, luciferase, chloramphenicol, and acetyltransferase. See Jefferson, R. A., Plant Mol. Biol. Rep., 5:387 (1987); Teeri et al., EMBO J., 8:343 (1989); Koncz et al., Proc. Natl. Acad. Sci. U.S.A., 84:131 (1987); and DeBlock et al., EMBO J., 3:1681 (1984). Another approach to identifying relatively rare transformation events has been the use of a gene that encodes a dominant constitutive regulator of the Zea mays anthocyanin pigmentation pathway. Ludwig et al., Science, 247:449 (1990).

The Green Fluorescent Protein (GFP) gene has been used as a marker for gene expression in prokaryotic and eukaryotic cells. See Chalfie et al., Science, 263:802 (1994). GFP and mutants of GFP may be used as screenable markers.

Genes included in expression vectors are driven by a nucleotide sequence comprising a regulatory element, for example, a promoter. Many suitable promoters are known in the art, as are other regulatory elements that may be used either alone or in combination with promoters.

As used herein, “promoter” refers to a region of DNA upstream or downstream from the transcription initiation site, a region that is involved in recognition and binding of RNA polymerase and other proteins to initiate transcription. A “plant promoter” is a promoter capable of initiating transcription in plant cells. Examples of promoters under developmental control include promoters that preferentially initiate transcription in certain tissues, such as leaves, roots, seeds, fibers, xylem vessels, tracheids, or sclerenchyma. Such promoters are referred to as “tissue-preferred.” Promoters that initiate transcription only in certain tissue are referred to as “tissue-specific.” A “cell type” specific promoter primarily drives expression in certain cell types in one or more organs, for example, vascular cells in roots or leaves. An “inducible” promoter is a promoter that is under environmental control. Examples of environmental conditions that may induce transcription by inducible promoters include anaerobic conditions or the presence of light. Tissue-specific, tissue-preferred, cell type specific, and inducible promoters are examples of “non-constitutive” promoters. A “constitutive” promoter is a promoter that is generally active under most environmental conditions.

A. Inducible Promoters

An inducible promoter is operably linked to a gene for expression in rice. Optionally, the inducible promoter is operably linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice. With an inducible promoter the rate of transcription increases in response to an inducing agent.

Any suitable inducible promoter may be used in the present invention. See Ward et al., Plant Mol. Biol., 22:361-366 (1993). Examples include those from the ACEI system, which responds to copper, Meft et al., PNAS, 90:4567-4571 (1993); In2 gene from maize, which responds to benzenesulfonamide herbicide safeners, Hershey et al., Mol. Gen Genetics, 227:229-237 (1991); Gatz et al., Mol. Gen. Genetics, 243:32-38 (1994); and Tet repressor from Tn10, Gatz, Mol. Gen. Genetics, 227:229-237 (1991). A preferred inducible promoter is one that responds to an inducing agent to which plants do not normally respond, for example, the inducible promoter from a steroid hormone gene, the transcriptional activity of which is induced by a glucocorticosteroid hormone. See Schena et al., Proc. Natl. Acad. Sci., U.S.A. 88:0421 (1991).

B. Constitutive Promoters

A constitutive promoter is operably linked to a gene for expression in rice, or the constitutive promoter is operably linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice.

Constitutive promoters may also be used in the instant invention. Examples include promoters from plant viruses such as the 35S promoter from cauliflower mosaic virus, Odell et al., Nature, 313:810-812 (1985), and the promoters from the rice actin gene, McElroy et al., Plant Cell, 2:163-171 (1990); ubiquitin, Christensen et al., Plant Mol. Biol., 12:619-632 (1989) and Christensen et al., Plant Mol. Biol. 18:675-689 (1992); pEMU, Last et al., Theor. Appl. Genet., 81:581-588 (1991); MAS, Velten et al., EMBO 1, 3:2723-2730 (1984); and maize H3 histone, Lepetit et al., Mol. Gen. Genetics, 231:276-285 (1992) and Atanassova et al., Plant Journal, 2 (3): 291-300 (1992). An ACCase or AHAS promoter, such as a rice ACCase or AHAS promoter, may be used as a constitutive promoter.

C. Tissue-Specific or Tissue-Preferred Promoters

A tissue-specific promoter is operably linked to a gene for expression in rice. Optionally, the tissue-specific promoter is operably linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice. Transformed plants produce the expression product of the transgene exclusively, or preferentially, in specific tissue(s).

Any tissue-specific or tissue-preferred promoter may be used in the instant invention. Examples of tissue-specific or tissue-preferred promoters include those from the phaseolin gene, Murai et al., Science, 23:476-482 (1983), and Sengupta-Gopalan et al., Proc. Natl. Acad. Sci. U.S.A., 82:3320-3324 (1985); a leaf-specific and light-induced promoter such as that from cab or rubisco, Simpson et al., EMBO J., 4(11):2723-2729 (1985) and Timko et al., Nature, 318:579-582 (1985); an anther-specific promoter such as that from LAT52, Twell et al., Mol. Gen. Genetics, 217:240-245 (1989); a pollen-specific promoter such as that from Zm13, Guerrero et al., Mol. Gen. Genetics, 244:161-168 (1993); or a microspore-preferred promoter such as that from apg, Twell et al., Sex. Plant Reprod, 6:217-224 (1993).

Signal Sequences for Targeting Proteins to Subcellular Compartments

Transport of protein or peptide molecules produced by transgenes to a subcellular compartment such as a chloroplast, vacuole, peroxisome, glyoxysome, cell wall, or mitochondrion, or for secretion into an apoplast, is accomplished by operably linking a nucleotide sequence encoding a signal sequence to the 5′ or 3′ end of a gene encoding the protein or peptide of interest. Targeting sequences at the 5′ or 3′ end of the structural gene may determine, during protein synthesis and processing, where the encoded protein is ultimately compartmentalized.

Many signal sequences are known in the art. See, for example, Becker et al., Plant Mol. Biol., 20:49 (1992); Close, P. S., Master's Thesis, Iowa State University (1993); Knox, C. et al., “Structure and Organization of Two Divergent Alpha-Amylase Genes from Barley,” Plant Mol. Biol., 9:3-17 (1987); Lerner et al., Plant Physiol., 91:124-129 (1989); Fontes et al., Plant Cell, 3:483-496 (1991); Matsuoka et al., Proc. Natl. Acad. Sci., 88:834 (1991); Gould et al., J. Cell. Biol., 108:1657 (1989); Creissen et al., Plant J., 2:129 (1991); Kalderon et al., “A short amino acid sequence able to specify nuclear location,” Cell, 39:499-509 (1984); and Steifel et al., “Expression of a maize cell wall hydroxyproline-rich glycoprotein gene in early leaf and root vascular differentiation,” Plant Cell, 2:785-793 (1990).

Foreign Protein Genes and Agronomic Genes

Agronomically significant genes that may be transformed into rice plants in accordance with the present invention include, for example, the following:

1. Genes that Confer Resistance to Pests or Disease:

-   -   A. Plant disease resistance genes. Plant defenses are often         activated by specific interaction between the product of a         disease resistance gene (R) in the plant and the product of a         corresponding avirulence (Avr) gene in the pathogen. A plant may         be transformed with a cloned resistance gene to engineer plants         that are resistant to specific pathogen strains. See, e.g.,         Jones et al., Science 266:789 (1994) (cloning of the tomato Cf-9         gene for resistance to Cladosporium fulvum); Martin et al.,         Science 262:1432 (1993) (tomato Pto gene for resistance to         Pseudomonas syringae pv. Tomato encodes a protein kinase); and         Mindrinos et al., Cell 78:1089 (1994) (Arabidopsis RSP2 gene for         resistance to Pseudomonas syringae).     -   B. A Bacillus thuringiensis protein, a derivative thereof, or a         synthetic polypeptide modeled thereon. See, e.g., Geiser et al.,         Gene 48:109 (1986), disclosing the cloning and nucleotide         sequence of a Bt-endotoxin gene. DNA molecules encoding         endotoxin genes may be obtained from American Type Culture         Collection, Manassas, Va., e.g., under ATCC Accession Nos.         40098, 67136, 31995, and 31998.     -   C. A lectin. See, for example, Van Damme et al., Plant Molec.         Biol. 24:25 (1994), disclosing the nucleotide sequences of         several Clivia miniata mannose-binding lectin genes.     -   D. A vitamin-binding protein such as avidin. See PCT Application         US93/06487. This disclosure teaches the use of avidin and avidin         homologues as larvicides against insect pests.     -   E. An enzyme inhibitor, e.g., a protease or proteinase inhibitor         or an amylase inhibitor. See, e.g., Abe et al., J. Biol. Chem.         262:16793 (1987) (nucleotide sequence of rice cysteine         proteinase inhibitor); Huub et al., Plant Molec. Biol.         21:985 (1993) (nucleotide sequence of cDNA encoding tobacco         proteinase inhibitor 1); and Sumitani et al., Biosci. Biotech.         Biochem. 57:1243 (1993) (nucleotide sequence of Streptomyces         nitrosporeus-amylase inhibitor).     -   F. An insect-specific hormone or pheromone such as an         ecdysteroid and juvenile hormone, a variant thereof, a mimetic         based thereon, or an antagonist or agonist thereof. See, e.g.,         Hammock et al., Nature, 344:458 (1990), disclosing baculovirus         expression of cloned juvenile hormone esterase, an inactivator         of juvenile hormone.     -   G. An insect-specific peptide or neuropeptide that, upon         expression, disrupts the physiology of the affected pest. See,         e.g., Regan, J. Biol. Chem. 269:9 (1994) (expression cloning         yields DNA coding for insect diuretic hormone receptor); and         Pratt et al., Biochem. Biophys. Res. Comm., 163:1243 (1989) (an         allostatin in Diploptera puntata). See also U.S. Pat. No.         5,266,317 to Tomalski et al., disclosing genes encoding         insect-specific, paralytic neurotoxins.     -   H. An insect-specific venom produced in nature by a snake, a         wasp, etc. For example, see Pang et al., Gene, 116:165 (1992),         concerning heterologous expression in plants of a gene coding         for a scorpion insectotoxic peptide.     -   An enzyme responsible for hyperaccumulation of a monoterpene, a         sesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid         derivative or another non-protein molecule with insecticidal         activity.     -   J. An enzyme involved in the modification, including         post-translational modification, of a biologically active         molecule; e.g., a glycolytic enzyme, a proteolytic enzyme, a         lipolytic enzyme, a nuclease, a cyclase, a transaminase, an         esterase, a hydrolase, a phosphatase, a kinase, a phosphorylase,         a polymerase, an elastase, a chitinase, or a glucanase, either         natural or synthetic. See PCT Application WO 9302197 to Scott et         al., which discloses the nucleotide sequence of a callase gene.         DNA molecules that contain chitinase-encoding sequences can be         obtained, for example, from the American Type Culture Collection         under Accession Nos. 39637 and 67152. See also Kramer et al.,         Insect Biochem. Molec. Biol. 23:691 (1993), which discloses the         nucleotide sequence of a cDNA encoding tobacco hookworm         chitinase; and Kawalleck et al., Plant Molec. Biol., 21:673         (1993), which discloses the nucleotide sequence of the parsley         ubi4-2 polyubiquitin gene.     -   K. A molecule that stimulates signal transduction. See, e.g.,         Botella et al., Plant Molec. Biol., 24:757 (1994), which         discloses nucleotide sequences for mung bean calmodulin cDNA         clones; and Griess et al., Plant Physiol., 104:1467 (1994),         which discloses the nucleotide sequence of a maize calmodulin         cDNA clone.     -   L. An antimicrobial or amphipathic peptide. See PCT Application         WO 9516776 (disclosing peptide derivatives of Tachyplesin that         inhibit fungal plant pathogens); and PCT Application WO 9518855         (disclosing synthetic antimicrobial peptides that confer disease         resistance).     -   M. A membrane permease, a channel former or a channel blocker.         See, e.g., Jaynes et al., Plant Sci., 89:43 (1993), which         discloses heterologous expression of a cecropin lytic peptide         analog to render transgenic tobacco plants resistant to         Pseudomonas solanacearum.     -   N. A viral-invasive protein or a complex toxin derived         therefrom. For example, the accumulation of viral coat proteins         in transformed plant cells induces resistance to viral infection         or disease development caused by the virus from which the coat         protein gene is derived, as well as by related viruses. Coat         protein-mediated resistance has been conferred upon transformed         plants against alfalfa mosaic virus, cucumber mosaic virus,         tobacco streak virus, potato virus X, potato virus Y, tobacco         etch virus, tobacco rattle virus, and tobacco mosaic virus. See         Beachy et al., Ann. Rev. Phytopathol., 28:451 (1990).     -   O. An insect-specific antibody or an immunotoxin derived         therefrom. Thus, an antibody targeted to a critical metabolic         function in the insect gut inactivates an affected enzyme,         killing the insect. See Taylor et al., Abstract #497, Seventh         Int'l Symposium on Molecular Plant-Microbe Interactions         (Edinburgh, Scotland, 1994) (enzymatic inactivation in         transgenic tobacco via production of single-chain antibody         fragments).     -   P. A virus-specific antibody. See, e.g., Tavladoraki et al.,         Nature, 366:469 (1993), showing protection of transgenic plants         expressing recombinant antibody genes from virus attack.     -   Q. A developmental-arrest protein produced in nature by a         pathogen or a parasite. For example, fungal         endo-1,4-D-polygalacturonases facilitate fungal colonization and         plant nutrient release by solubilizing plant cell wall         homo-1,4-D-galacturonase. See Lamb et al., Bio/Technology,         10:1436 (1992). The cloning and characterization of a gene that         encodes a bean endopolygalacturonase-inhibiting protein is         described by Toubart et al., Plant 1, 2:367 (1992).     -   R. A developmental-arrest protein produced in nature by a plant.         For example, Logemann et al., Bio/Technology, 10:305 (1992)         reported that transgenic plants expressing the barley         ribosome-inactivating gene have an increased resistance to         fungal disease.

2. Genes that Confer Additional Resistance to a Herbicide, Beyond that which is Inherent in ‘CLJ01,’ for Example:

-   -   A. A herbicide that inhibits the growing point or meristem, such         as an imidazolinone or a sulfonylurea. Exemplary genes in this         category code for mutant ALS and AHAS enzymes as described, for         example, by Lee et al., EMBO 1, 7:1241 (1988); and Miki et al.,         Theor. Appl. Genet., 80:449 (1990), respectively. See,         additionally, U.S. Pat. Nos. 5,545,822; 5,736,629; 5,773,703;         5,773,704; 5,952,553; 6,274,796; 6,943,280; 7,019,196;         7,345,221; 7,399,905; 7,495,153; 7,754,947; 7,786,360;         8,841,525; 8,841,526; 8,946,528; 9,029,642; 9,090,904; and         9,220220. Resistance to AHAS-acting herbicides may be through a         mechanism other than a resistant AHAS enzyme. See, e.g., U.S.         Pat. No. 5,545,822.     -   B. Glyphosate: Resistance may be imparted by mutant         5-enolpyruvl-3-phosphikimate synthase (EPSP) and aroA genes.         Other phosphono compounds such as glufosinate: Resistance may be         imparted by phosphinothricin acetyl transferase, PAT and         Streptomyces hygroscopicus phosphinothricin-acetyl transferase,         bar, genes. Pyridinoxy or phenoxy propionic acids and         cyclohexones: Resistance may be imparted by ACCase         inhibitor-encoding genes. See, e.g., U.S. Pat. No. 4,940,835 to         Shah et al., which discloses the nucleotide sequence of a form         of EPSP that confers glyphosate resistance. A DNA molecule         encoding a mutant aroA gene can be obtained under ATCC Accession         Number 39256, and the nucleotide sequence of the mutant gene is         disclosed in U.S. Pat. No. 4,769,061 to Comai. European Patent         Application No. 0333033 to Kumada et al.; and U.S. Pat. No.         4,975,374 to Goodman et al., disclose nucleotide sequences of         glutamine synthetase genes that confer resistance to herbicides         such as L-phosphinothricin. The nucleotide sequence of a         phosphinothricin-acetyltransferase gene is provided in European         Application No. 0242246 to Leemans et al. and DeGreef et al.,         Bio/Technology, 7:61 (1989), describing the production of         transgenic plants that express chimeric bar genes coding for         phosphinothricin acetyl transferase activity. Examples of genes         conferring resistance to phenoxy propionic acids and         cyclohexones, such as sethoxydim and haloxyfop, are the Accl-S1,         Accl-S2, and Accl-S3 genes described by Marshall et al., Theor.         Appl. Genet., 83:435 (1992).     -   C. A herbicide that inhibits photosynthesis, such as a triazine         (psbA and gs+ genes) or a benzonitrile (nitrilase gene).         Przibilla et al., Plant Cell, 3:169 (1991), describe the         transformation of Chlamydomonas with plasmids encoding mutant         psbA genes. Nucleotide sequences for nitrilase genes are         disclosed in U.S. Pat. No. 4,810,648 to Stalker, and DNA         molecules containing these genes are available under ATCC         Accession Nos. 53435, 67441, and 67442. Cloning and expression         of DNA coding for a glutathione S-transferase is described by         Hayes et al., Biochem. 1, 285:173 (1992).

3. Genes that Confer or Contribute to a Value-added Trait, such as:

-   -   A. Modified fatty acid metabolism, for example, by transforming         a plant with an antisense sequence to stearyl-ACP desaturase, to         increase stearic acid content of the plant. See Knultzon et al.,         Proc. Natl. Acad. Sci. U.S.A. 89:2624 (1992).     -   B. Decreased Phytate Content         -   1) Introduction of a phytase-encoding gene would enhance             breakdown of phytate, adding more free phosphate to the             transformed plant. See, e.g., Van Hartingsveldt et al.,             Gene, 127:87 (1993), which discloses the nucleotide sequence             of an Aspergillus niger phytase gene.         -   2) A gene may be introduced to reduce phytate content. For             example, this may be accomplished by cloning, and then             reintroducing DNA associated with an allele that is             responsible for maize mutants characterized by low levels of             phytic acid, or a homologous or analogous mutation in rice             may be used. See Raboy et al., Maydica, 35:383 (1990).     -   C. Carbohydrate composition may be modified, for example, by         transforming plants with a gene coding for an enzyme that alters         the branching pattern of starch. See Shiroza et al., J.         Bacteol., 170:810 (1988) (nucleotide sequence of Streptococcus         mutant fructosyltransferase gene); Steinmetz et al., Mol. Gen.         Genet., 20:220 (1985) (nucleotide sequence of Bacillus subtilis         levansucrase gene); Pen et al., Bio/Technology, 10:292 (1992)         (production of transgenic plants that express Bacillus         lichenifonnis amylase); Elliot et al., Plant Molec. Biol.,         21:515 (1993) (nucleotide sequences of tomato invertase genes);         Sogaard et al., J. Biol. Chem., 268:22480 (1993) (site-directed         mutagenesis of barley amylase gene); and Fisher et al., Plant         Physiol., 102:1045 (1993) (maize endosperm starch branching         enzyme 11).

Methods for Rice Transformation

Numerous methods for plant transformation are known in the art, including both biological and physical transformation protocols. See, e.g., Miki, et al., “Procedures for Introducing Foreign DNA into Plants” in Methods in Plant Molecular Biology and Biotechnology; Glick B. R. and Thompson, J. E. (Eds.) (CRC Press, Inc., Boca Raton, 1993), pp. 67-88. In addition, expression vectors and in vitro culture methods for plant cell or tissue transformation and regeneration of plants are known in the art. See, e.g., Gruber et al., “Vectors for Plant Transformation” in Methods in Plant Molecular Biology and Biotechnology, Glick B. R. and Thompson, J. E. (Eds.) (CRC Press, Inc., Boca Raton, 1993), pp. 89-119.

A. Agrobacterium-Mediated Transformation

One method for introducing an expression vector into plants is based on the natural transformation system of Agrobacterium. See, e.g., Horsch et al., Science, 227:1229 (1985). A. tumefaciens and A. rhizogenes are plant pathogenic soil bacteria that genetically transform plant cells. The Ti and Ri plasmids of A. tumefaciens and A. rhizogenes, respectively, carry genes responsible for genetic transformation of plants. See, e.g., Kado, C. I., Crit. Rev. Plant Sci., 10:1 (1991). Descriptions of Agrobacterium vector systems and methods for Agrobacterium-mediated gene transfer are provided by Gruber et al., supra; Miki et al., supra; and Moloney, et al., Plant Cell Reports, 8:238 (1989). See also U.S. Pat. No. 5,591,616.

B. Direct Gene Transfer

Despite the fact the host range for Agrobacterium-mediated transformation is broad, it is more difficult to transform some cereal crop species and gymnosperms via this mode of gene transfer, although success has been achieved in both rice and corn. See Hiei et al., The Plant Journal, 6:271-282 (1994); and U.S. Pat. No. 5,591,616. Other methods of plant transformation exist as alternatives to Agrobacterium-mediated transformation.

A generally applicable method of plant transformation is microprojectile-mediated (so-called “gene gun”) transformation, in which DNA is carried on the surface of microprojectiles, typically 1 to 4 μm in diameter. The expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to typical speeds of 300 to 600 m/s, sufficient to penetrate plant cell walls and membranes. Sanford et al., Part. Sci. Technol., 5:27 (1987); Sanford, J. C., Trends Biotech., 6:299 (1988); Klein et al., Bio/Technology, 6:559-563 (1988); Sanford, J. C., Physiol Plant, 7:206 (1990); and Klein et al., Biotechnology, 10:268 (1992). Various target tissues may be bombarded with DNA-coated microprojectiles to produce transgenic plants, including, for example, callus (Type I or Type II), immature embryos, and meristematic tissue.

Another method for physical delivery of DNA to plants is sonication of target cells. Zhang et al., Bio/Technology, 9:996 (1991). Alternatively, liposome or spheroplast fusion has been used to introduce expression vectors into plants. Deshayes et al., EMBO J., 4:2731 (1985); and Christou et al., Proc Natl. Acad. Sci. U.S.A., 84:3962 (1987). Direct uptake of DNA into protoplasts, using CaCl₂) precipitation, polyvinyl alcohol or poly-L-ornithine, has also been reported. Hain et al., Mol. Gen. Genet., 199:161 (1985); and Draper et al., Plant Cell Physiol., 23:451 (1982). Electroporation of protoplasts and whole cells and tissues has also been described. Donn et al., in Abstracts of VIIth International Congress on Plant Cell and Tissue Culture IAPTC, A2-38, p. 53 (1990); D'Halluin et al., Plant Cell, 4:1495-1505 (1992); and Spencer et al., Plant Mol. Biol., 24:51-61 (1994).

Following transformation of rice target tissues, expression of a selectable marker gene allows preferential selection of transformed cells, tissues, or plants, using regeneration and selection methods known in the art.

These methods of transformation may be used for producing a transgenic inbred line. The transgenic inbred line may then be crossed with another inbred line (itself either transformed or non-transformed), to produce a new transgenic inbred line. Alternatively, a genetic trait that has been engineered into a particular rice line may be moved into another line using traditional crossing and backcrossing techniques. For example, backcrossing may be used to move an engineered trait from a public, non-elite inbred line into an elite inbred line, or from an inbred line containing a foreign gene in its genome into an inbred line or lines that do not contain that gene.

The term “inbred rice plant” should be understood also to include single gene conversions of an inbred line. Backcrossing methods can be used with the present invention to improve or introduce a characteristic into an inbred line.

Many single gene traits have been identified that are not regularly selected for in the development of a new inbred line, but that may be improved by crossing and backcrossing. Single gene traits may or may not be transgenic. Examples of such traits include male sterility, waxy starch, herbicide resistance, resistance for bacterial or fungal or viral disease, insect resistance, male fertility, enhanced nutritional quality, yield stability, and yield enhancement. These genes are generally inherited through the nucleus. Known exceptions to the nuclear genes include some genes for male sterility that are inherited cytoplasmically, but that still act functionally as single gene traits. Several single gene traits are described in U.S. Pat. Nos. 5,777,196; 5,948,957; and 5,969,212.

DEPOSIT INFORMATION

A sample of the rice cultivar designated ‘CLJ01’ was deposited with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209 on 5 Apr. 2018, and was assigned ATCC Accession No. PTA-125053. This deposit was made under the Budapest Treaty.

MISCELLANEOUS

The complete disclosures of all references cited in this specification are hereby incorporated by reference. In the event of an otherwise irreconcilable conflict, however, the present specification shall control. 

What is claimed:
 1. A rice plant of the variety ‘CLJ01,’ a representative sample of seeds of said variety having been deposited under ATCC Accession No. PTA-125053; or an F₁ hybrid of the variety ‘CLJ01,’ wherein said F₁ hybrid expresses the AHAS herbicide resistance characteristics of ‘CLJ01.’
 2. The rice plant of claim 1, wherein said rice plant is a rice plant of the variety ‘CLJ01.’
 3. Rice seed of the rice plant of claim 2, or rice seed capable of producing said rice plant.
 4. The rice plant of claim 1, wherein said rice plant is an F₁ hybrid of the variety ‘CLJ01,’ and wherein said rice plant expresses the aromatic cooking characteristics of ‘CLJ01.’
 5. Rice seed capable of producing the rice plant of claim
 4. 6. Rice seed of the rice plant of claim 1, or rice seed capable of producing said rice plant.
 7. The seed of claim 6, wherein said seed is treated with an AHAS-inhibiting herbicide.
 8. The seed of claim 7, wherein the AHAS-inhibiting herbicide comprises an imidazolinone herbicide.
 9. The seed of claim 7, wherein the AHAS-inhibiting herbicide comprises a sulfonylurea herbicide.
 10. The seed of claim 6, wherein said seed is coated with a seed treatment or contains a seed treatment.
 11. Pollen of the plant of claim
 1. 12. An ovule of the plant of claim
 1. 13. A composition comprising a product prepared from the rice plant of claim
 1. 14. A tissue culture of regenerable cells or protoplasts produced from the rice plant of claim
 1. 15. The tissue culture of claim 14, wherein said regenerable cells or protoplasts are produced from a tissue selected from the group consisting of embryos, meristematic cells, pollen, leaves, anthers, roots, root tips, flowers, seeds, and stems.
 16. A method for producing rice plants, said method comprising planting a plurality of rice seeds of the rice plant of claim 1, or a plurality of rice seeds capable of producing said rice plant, under conditions favorable for the growth of rice plants.
 17. The method of claim 16, additionally comprising the step of applying herbicide in the vicinity of the rice plants, wherein the herbicide normally inhibits acetohydroxyacid synthase, at levels of the herbicide that would normally inhibit the growth of a rice plant.
 18. The method of claim 17, additionally comprising the step of producing rice seed from the resulting rice plants.
 19. Rice seed produced by the method of claim
 18. 20. The method of claim 17, wherein the herbicide comprises an imidazolinone herbicide.
 21. The method of claim 20, wherein the herbicide comprises imazethapyr or imazamox.
 22. The method of claim 17, wherein the herbicide comprises a sulfonyl herbicide.
 23. A method of producing an insect-resistant rice plant, said method comprising transforming the rice plant of claim 1 with one or more of: a transgene that confers insect resistance, a transgene that confers disease resistance, a transgene that encodes fructosyltransferase, a transgene that encodes levansucrase, a transgene that encodes alpha-amylase, a transgene that encodes invertase, a transgene that encodes a starch-branching enzyme, or a transgene that encodes an antisense sequence to stearyl-ACP desaturase.
 24. A rice plant or rice seed produced by the method of claim
 23. 25. A method of introducing a desired trait into rice cultivar ‘CLJ01,’ said method comprising the steps of: (a) crossing plants as recited in claim 1 with plants of another rice line expressing the desired trait, to produce progeny plants; (b) selecting progeny plants that express the desired trait, to produce selected progeny plants; (c) crossing the selected progeny plants with plants as recited in claim 1 to produce new progeny plants; (d) selecting new progeny plants that express both the desired trait and some or all of the physiological and morphological characteristics of rice cultivar ‘CLJ01,’ to produce new selected progeny plants; and (e) repeating steps (c) and (d) three or more times in succession, to produce selected higher generation backcross progeny plants that express both the desired trait and essentially all of the physiological and morphological characteristics of rice cultivar ‘CLJ01,’ as described in the VARIETY DESCRIPTION INFORMATION of the specification, determined at a 5% significance level, when grown in the same environmental conditions; wherein the selected plants express the AHAS herbicide resistance of ‘CLJ01,’ and wherein the selected plants express the aromatic cooking characteristics of ‘CLJ01.’
 26. Rice seed from a progeny plant produced by the method of claim 25; wherein, if said rice seed is grown, then the rice plants grown from said rice seed will express the AHAS herbicide resistance characteristics and the aromatic cooking characteristics of ‘CLJ01.’
 27. The method of claim 25, additionally comprising the step of planting a plurality of rice seed produced by selected higher generation backcross progeny plants under conditions favorable for the growth of rice plants.
 28. The method of claim 27, additionally comprising the step of applying herbicide in the vicinity of the rice plants, wherein the herbicide normally inhibits acetohydroxyacid synthase, at levels of the herbicide that would normally inhibit the growth of a rice plant.
 29. The method of claim 28, wherein the herbicide comprises an imidazolinone herbicide.
 30. The method of claim 29, wherein the herbicide comprises imazethapyr or imazamox.
 31. The method of claim 29, wherein the herbicide comprises a sulfonylurea herbicide.
 32. The method of claim 25, wherein the selected progeny plants are F₁ hybrid plants. 